Isolation and characterization of human cord blood mesenchymal stem cells multipotent clones / (Record no. 61503)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03646cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223031742.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 170709s2016 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.11.07.Ph.D.2016.No.I |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Noha Mohamed Abdelrahman Ali |
| 245 10 - TITLE STATEMENT | |
| Title | Isolation and characterization of human cord blood mesenchymal stem cells multipotent clones / |
| Statement of responsibility, etc. | Noha Mohamed Abdelrahman Ali ; Supervised Shereen Mahmoud Kamel , Zeinab AbouBakr Demerdash , Rania Hassan Khalifa |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | {uFED3}{uFEBB}{uFEDE} و {uئإ٩٧}{uئإإ٣}{uئآئإ}{uئآئإ}ز ا{uئإؤئ}{uئإإ٣}{uئإآ٣}{uئإ٩٧}{uئإإ٧}{uئإآ٣}{uئإء٧}{uئإ٨إ}ت {uئإإ٣}{uئإ٩٧}{uئإأأ}ددة ا{uئإؤئ}{uئإؤ٨}وى ا{uئإؤئ}{uئإإ٣}{uئإآ٧}{uئإ٩٧}{uئإؤ٨}{uئإ٩٤} {uئإإ٣}ن ا{uئإؤئ}{uئإء٧}{uئإئأ}يا ا{uئإؤئ}{uئإ٩ئ}ذ{uئإأآ}ية ا{uئإؤئ}{uئإإ٣}{uئآئإ}ز{uئإإ٧}{uئإآ٧}ية {uئإإ٣}ن دم ا{uئإؤئ}{uئإء٣}{uئإ٩١}{uئإؤإ} ا{uئإؤئ}{uئإآ٣}رى ا{uئإئ٥}دمى |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Noha Mohamed Abdelrahman Ali , |
| Date of publication, distribution, etc. | 2016 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 157 P. : |
| Other physical details | charts , facsimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Background: Mesenchymal stem cells (MSCs) are self-renewing cells that have extensive capacity for in vitro expansion with high yield and immunomodulatory effects, that's why they are excellent choice in regenerative medicine. However, clonal analysis of these cells has revealed that they are a heterogeneous mixture of cells. Therefore, clones derived from a single cell would provide a homogeneous population of MSCs and therapeutic efficacy of MSCs population may be enhanced by the use of selected multilineage clones. Aim of the work: Cloning of umbilical cord blood MSCs, by limiting dilution method, could provide a better source for MSCs with high stemness calibre to meet clinical demands. Methodology: Isolation, expansion and cloning by limiting dilution method of human umbilical cord blood MSCs were done. Two cord blood samples, mother (A) and mother (B) were compared with their corresponding daughter clones regarding their proliferation efficiency, surface antigen expression, pluripotent and proliferation genes expression, as well as, their differentiation potentials. Results: MSCs were successfully isolated from two cord blood samples (Mother (A) and Mother (B)) and cloned by limiting dilution method to give 7 single clones for Mother (A) and 8 single clones for Mother (B). Mother (B) was fast growing, with higher PD and shorter PDT than Mother (A). It showed higher pluripotency and proliferation genes expression, as well as, a higher differentiation percent on the three mesodermal lineages level. The daughter clones (A2, A5) had a higher stemness criteria than their mother (A) and other clones, whereas, mother (B) showed a higher stemness characteristics than its daughter clones, except for clone B6 which showed comparable result to its mother |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Cloning |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Limiting dilution method |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Mesenchymal stem cells |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Rania Hassan Khalifa , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Shereen Mahmoud Kamel , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zeinab AbouBakr Demerdash , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Samia |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.11.07.Ph.D.2016.No.I | 01010110072070000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.11.07.Ph.D.2016.No.I | 01020110072070000 | 22.09.2023 | CD - Rom | 72070.CD |