MARC details
| 000 -LEADER |
|---|
| fixed length control field |
03229cam a2200325 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
EG-GiCUC |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
170712s2016 ua h f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
EG-GiCUC |
| Language of cataloging |
eng |
| Transcribing agency |
EG-GiCUC |
| 041 0# - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| 049 ## - LOCAL HOLDINGS (OCLC) |
|---|
| Holding library |
Deposite |
| 097 ## - Thesis Degree |
|---|
| Thesis Level |
M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) |
|---|
| Classification number |
Cai01.12.21.M.Sc.2016.Ra.I |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Ramy Abdelnaby Gadalla |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Isolation and characterization of tumor-associated plasmacytoid dendritic cells in breast cancer patients / |
| Statement of responsibility, etc. |
Ramy Abdelnaby Gadalla ; Supervised Somaya Osman Eldeeb , Mona Mostafa Mohamed , Mohamed Elsayed Elshinawi |
| 246 15 - VARYING FORM OF TITLE |
|---|
| Title proper/short title |
عزل و توصيف الخلايا الشجيرية البلازماوية المصاحبة للورم فى مرضى سرطان الثدى |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
|---|
| Place of publication, distribution, etc. |
Cairo : |
| Name of publisher, distributor, etc. |
Ramy Abdelnaby Gadalla , |
| Date of publication, distribution, etc. |
2016 |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
136 P. : |
| Other physical details |
facsimiles ; |
| Dimensions |
25cm |
| 502 ## - DISSERTATION NOTE |
|---|
| Dissertation note |
Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Zoology |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
Elevated plamsacytoid dendritic cells (PDCs) infiltration into breast cancer tissues has been reported to be associated with poor prognosis, shorter overall and relapse-free survival rate. CXCR4/SDF-1 axis is a central chemokine axis contributing to lymph node metastasis in breast cancer. The aim of the present study was to investigate whether PDCs may contribute to lymph node metastasis through CXCR4 and to identify the underlying molecular mechanisms. We assessed PDCs infiltration into breast cancer tissues of positive lymph node (pLN) and negative lymph nodes (nLN) patients by immunostaining of CD303+ cells. In addition, CXCR4+cells percentages in breast carcinoma tissues were counted by flow cytometry. PDCs were immuno-magnetically isolated from axillary tributaries during modified radical mastectomy and cultured overnight to produce media conditioned by PDCs secrotome. The human breast cancer cell lines SKBR3 and MCF-7 were employed to test the effect of PDCs secretome on CXCR4 expression. Also, we quantified the expression of SDF-1 in lymph nodes of both groups. We found that breast carcinoma tissues of pLN breast cancer patients have higher PDCs infiltration than those of nLN ones. Also, we showed that the breast cancer tissues of pLN patients encompasses more CXCR4+cells than those of nLN patients. Interestingly, we found that SDF-1 is highly expressed on lymph nodes metastases. Quantitative PCR results indicated a significant upregulation of mRNA expression of TNF-Ü in pLN patients' PDCs. Mechanistically, expression of CXCR4 was upregulated, in parallel with NF-{u03F0}B upregulation, in MCF-7 and SKBR-3 cell lines upon stimulation with secretome of PDCs isolated from pLN patients compared to nLN patients. In conclusion, our study suggests a potential role for PDCs in lymph node metastasis via CXCR4/SDF-1 axis, which could be therapeutically targeted to hamper breast cancer metastasis |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE |
|---|
| Additional physical form available note |
Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
Breast cancer |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
CXCR4 |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
Plasmacytoid dendritic cells |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Mohamed Elsayed Elshinawi , |
| Relator term |
|
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Mona Mostafa Mohamed , |
| Relator term |
|
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Somaya Osman Eldeeb , |
| Relator term |
|
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
|---|
| Cataloger |
Nazla |
| Reviser |
Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
|---|
| Cataloger |
Samia |
| Reviser |
Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Koha item type |
Thesis |
