MARC details
| 000 -LEADER |
|---|
| fixed length control field |
03102cam a2200325 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
EG-GiCUC |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
170911s2016 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
EG-GiCUC |
| Language of cataloging |
eng |
| Transcribing agency |
EG-GiCUC |
| 041 0# - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| 049 ## - LOCAL HOLDINGS (OCLC) |
|---|
| Holding library |
Deposite |
| 097 ## - Thesis Degree |
|---|
| Thesis Level |
M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) |
|---|
| Classification number |
Cai01.12.25.M.Sc.2016.Om.P |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Omar Ahmed Khaled Taha Ahmed Eldahshoury |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Punica granatum extract as a regulator of low-density lipoprotein receptor (LDLR) and HMG CoA reductase gene expression in hepG2-cell line / |
| Statement of responsibility, etc. |
Omar Ahmed Khaled Taha Ahmed Eldahshoury ; Supervised Magda Ahmed Abdallah , Alaa Ahmed Barakat , Heba Mohamed Kamal |
| 246 15 - VARYING FORM OF TITLE |
|---|
| Title proper/short title |
فى خلايا الكبد السرطانية (LDLR) و مستقبلات البروتينات الدهنية منخفضة الكثافة (HMG CoA reductase) مستخلص الرمان كمنظم طبيعى للتعبير الجينى لانزيم مختزل الهيدروميثيل جلوتاريل كو - أيه |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
|---|
| Place of publication, distribution, etc. |
Cairo : |
| Name of publisher, distributor, etc. |
Omar Ahmed Khaled Taha Ahmed Eldahshoury , |
| Date of publication, distribution, etc. |
2016 |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
119 P. : |
| Other physical details |
charts , facsimiles ; |
| Dimensions |
25cm |
| 502 ## - DISSERTATION NOTE |
|---|
| Dissertation note |
Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Biotechnology |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
Objective: Pomegranate (punica granatum L., Lythraceae) peel has concentrated amounts of lipid-lowering elements that demonstrated, in various hoary and recent studies, their effects against obesity and hyperlipidemia, involving elevated rates of lipid and lipoprotein levels in blood and increases risking of cardiovascular diseases and other diseases. We aim to study expression modulation of genes involved in lipid metabolism by impact of standardized pomegranate peel extract (PPE) in a comprehensive research on human liver cells and experimental mice. Methods: Initially, after extraction process, reversed-phase HPLC was used to determine amounts of ellagic acid as a standard phytochemical. And then, using reverse-transcription real-time PCR, an in-vitro study harnessing HepG2 cell-line was conducted to determine the hyperlipidemia-related gene expression profiles and cytotoxic effects upon treatment with PPE. In another complementary in-vivo study, male C57BL/6J mice were fed a high-fat diet (HFD) or an HFD supplemented with PPE for 14 days to define expression of lipid-metabolism related genes that control obesity. Correspondingly, DNA fragmentation effect and fatty liver proportions were also estimated after treatment. Results: Higher mRNA expression of LDL receptor (LDL-R) and down-regulation of sterol regulatory element-binding protein (SREBF-2), (SRBEP-1c), Fatty acid synthase (FAS) and 3-hydroxy-3- methylglutaryl-CoA reductase (HMGCR) upon PPE treatment in HepG2 cell-line were significantly recorded analysis. |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE |
|---|
| Additional physical form available note |
Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
Hyperlipidemia |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
Peel extract |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
Punica granatum L. |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Alaa Ahmed Barakat , |
| Relator term |
|
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Heba Mohamed Kamal , |
| Relator term |
|
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Magda Ahmed Abdallah , |
| Relator term |
|
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
|---|
| Cataloger |
Nazla |
| Reviser |
Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
|---|
| Cataloger |
Samia |
| Reviser |
Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Koha item type |
Thesis |
