Generation of transgene-free induced pluripotent stem cells from cardiac fibroblasts of goat embryos / (Record no. 63004)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02877cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223031827.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 171017s2017 ua dho f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.11.30.Ph.D.2017.Mi.G |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Mira Barsoum Nashid Barsoum Hanna |
| 245 10 - TITLE STATEMENT | |
| Title | Generation of transgene-free induced pluripotent stem cells from cardiac fibroblasts of goat embryos / |
| Statement of responsibility, etc. | Mira Barsoum Nashid Barsoum Hanna ; Supervised Moshira Rateb , Konrad Brockmeier , Hala Gabr |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | إنتاج خلايا جذعية محفزة خالية من التحوير من الخلايا الليفية المأخوذة من أنسجة عضلة قلب أجنة الماعز |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Mira Barsoum Nashid Barsoum Hanna , |
| Date of publication, distribution, etc. | 2017 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 221 P. : |
| Other physical details | charts , facsimiles , photographs ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Physiology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Induced pluripotent stem cells (iPSCs) generation hold a great potential for therapeutic regenerative medicine. Since its production by takahashi and yamanaka 2006, the researchers are trying to find the best method to reprogram the adult somatic cells to be suitable for transplantation. A lot of promises have been settled in the field of chronic life threatening worldwide diseases such as cardiac diseases and diabetes. The aim of this study was to generate induced pluripotent stem (iPS) cells from goat embryonic cardiac tissue derived fibroblasts. The iPS cells, generated in the frame of this approach were produced without the use of integrating virus and reprogramming transgenes were removed at the end of the process. Transgene-free iPS cells were in vitro assayed for pluripotency. It can be thus concluded that we succeeded to isolate cardiac fibroblasts from cardiac tissue of goat embryos that were alfa smooth muscle actin, vimentin and discoidin doman receptor2 positive. From these cells, we generated transgene free iPSCs using piggyBac transposons / transposase using five transcription factors (Oct4, Sox2, KLF, Myc and Lin 28). They were SSEA1, SSEA4 and Oct4 positive. They were cultured on neofeeders using 20% Serum replacement-IMDM with bFGF. They could form cystic and compact embryoid bodies that showed differentiated ectodermal and mesodermal like cells when cultured using 20% FBS-IMDM without bFGF |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Cardiac fibroblast |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Induced pluripotent stem cells |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Transfection methods |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hala Gabr , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Konrad Brockmeier , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Moshira Rateb , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Samia |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.11.30.Ph.D.2017.Mi.G | 01010110073110000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.11.30.Ph.D.2017.Mi.G | 01020110073110000 | 22.09.2023 | CD - Rom | 73110.CD |