Studying the role of cytokines in transactivation of the growth factor receptors signaling pathways / (Record no. 63480)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03238cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223031842.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 171114s2017 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.12.25.M.Sc.2017.Sh.S |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Shaza Ahmed Habib |
| 245 10 - TITLE STATEMENT | |
| Title | Studying the role of cytokines in transactivation of the growth factor receptors signaling pathways / |
| Statement of responsibility, etc. | Shaza Ahmed Habib ; Supervised Mohamed Ahmed Badawy , Mona Mostafa Mohamed , Ayman Ali Diab |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | دراسة عن دور الستوكينات في التنشيط غير المباشر للمسارات الخلوية لمستقبلات عوامل النمو |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Shaza Ahmed Habib , |
| Date of publication, distribution, etc. | 2017 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 141 P. : |
| Other physical details | charts , facsimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Biotechnology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | The epidermal growth factor receptor (EGFR) and EGFR2 are members of ErbB family receptor tyrosine kinases (RTK{u2019}s), which are frequently overexpressed in breast cancer and thus have been validated as targets for tyrosine kinase inhibitors such as lapatinib drug. However, transactivation of the EGFR and EGFR2 tyrosine residues could be utilized via different cytokines stimulation such as interleukin-6 or -8 (IL-6 or IL-8), suppressing the effect of tyrosine kinase inhibitors. In the present study, we evaluated the effect of IL-6 and IL-8 (50 ng/ml and 100 ng/ml) on activation of the EGFR and EGFR2 in the SKBR3 breast cancer cell line. Moreover, we assessed the impact of IL-6 and IL-8-induced activation of the EGFR, EGFR2, ERK1/2, STAT3 and non-receptor tyrosine kinases signaling molecule Src by Western blot, 3D-spheroids formation and the invasive potential using BD Bio-Coat Matrigel Invasion chamber in the absence and presence of lapatanib drug. Our data indicated that the IL-6 and IL-8 transactivated the EGFR, EGFR2 at Tyr-845 and Tyr-Y1248 tyrosine kinases respectively upon stimulation for the SKBR3 breast cancer cell line. Interestingly, our results showed that IL-6 and IL-8-induced activation of EGFR and EGFR2 downstream signaling clues including (Src, ERK1/2 and STAT3). Whereby the lapatinib drug did not inhibit the signaling molecules stimulated by IL-6 and IL-8 at the specified time intervals when compared to control cells. On the other hand, the increase in number and size of tumor spheroid formation by SKBR3 is an indicator reflecting the impact of IL-6 and IL-8 in increasing the cell proliferation, in the presence of lapatinib drug when compared to control cells seeded in complete medium where the spheroid formation increased upon stimulation. On the other hand, the drug have shown to be lethal to control cells in the absence of stimulants |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Breast cancer |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | EGFR |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | EGFR2 |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ayman Ali Diab , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mohamed Ahmed Badawy , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mona Mostafa Mohamed , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Shimaa |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.12.25.M.Sc.2017.Sh.S | 01010110073318000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.12.25.M.Sc.2017.Sh.S | 01020110073318000 | 22.09.2023 | CD - Rom | 73318.CD |