MARC details
| 000 -LEADER |
|---|
| fixed length control field |
03157cam a2200313 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
EG-GiCUC |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
171125s2017 ua o f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
EG-GiCUC |
| Language of cataloging |
eng |
| Transcribing agency |
EG-GiCUC |
| 041 0# - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| 049 ## - LOCAL HOLDINGS (OCLC) |
|---|
| Holding library |
Deposite |
| 097 ## - Thesis Degree |
|---|
| Thesis Level |
M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) |
|---|
| Classification number |
Cai01.10.09.M.Sc.2017.Sa.S |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Samah Elsayed Mahmoud Elsaman |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Studies on lumpy skin disease in cattle / |
| Statement of responsibility, etc. |
Samah Elsayed Mahmoud Elsaman ; Supervised Magdy Mahmoud Elsayed , Hassan Mohamed Elsaeid |
| 246 15 - VARYING FORM OF TITLE |
|---|
| Title proper/short title |
دراسات عن مرض الجلد العقدى فى الابقار |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
|---|
| Place of publication, distribution, etc. |
Cairo : |
| Name of publisher, distributor, etc. |
Samah Elsayed Mahmoud Elsaman , |
| Date of publication, distribution, etc. |
2017 |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
94 P. : |
| Other physical details |
photographs ; |
| Dimensions |
25cm |
| 502 ## - DISSERTATION NOTE |
|---|
| Dissertation note |
Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Internal Medicine and Infectious Diseases |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
Lumpy skin disease (LSD) is an acute to chronic, highly infectious, generalized skin disease of cattle. The disease considered to be the most serious disease of cattle caused by lumpy skin disease virus (LSDV) type (Neethling strain) similar to that causing sheep pox and goat pox, and is transmitted mostly by biting insects. It considered a ''List A'' disease by the office International des epizooties (OIE) due to it's potential for rapid spread and ability to cause severe economic losses. The disease affects cattle breeds derived from both Bos taurus and Bos indicus. In the current study, a total number of 77 skin nodules and scabs were collected from clinically diseased cattle showing signs typical to lumpy skin disease for laboratory investigation. A molecular diagnosis of the disease have been done including simple conventional PCR assay on all 77 skin samples using a universal primers, the results indicated that the positive samples (61/77) followed by virus isolation on ECE the results (18/61) were positive. The isolated samples on CAM were confirmed by PCR assay using primers specific for LSD virus (gpcr) gene. One representative positive sample was sequenced and analyzed by a phylogenetic analysis. The result revealed that the virus isolate from diseased cattle in Egypt was identical to LSD strain of turkey and kenyan strain ks-1 than LSD Ismailia/89 strain. A transmission electron microscopy (TEM) was performed on the positive PCR and sequenced sample for further confirmatory diagnosis. After confirmation, the viral isolate was propagated on cell cultures for 20 times on primary lamb heart and kidney cells and for 40 times on MDBK cell line to prepare an attenuated strain of LSDV from local isolates. The safety and sterility of attenuated virus was tested in cattle by 103.5and 105.5 /animal, the results indicated that the attenuated virus is safe and sterile. The results indicated that the virus candidate could be used as a vaccine for cattle rather than sheep pox vaccine |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE |
|---|
| Additional physical form available note |
Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
Cattle |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
LSD |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
Lumpy skin disease (LSD) |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Hassan Mohamed Elsaeid , |
| Relator term |
|
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Magdy Mahmoud Elsayed , |
| Relator term |
|
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
|---|
| Cataloger |
Nazla |
| Reviser |
Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
|---|
| Cataloger |
Samia |
| Reviser |
Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Koha item type |
Thesis |
