Characterization of surface markers of salivary gland stem cells versus periodontal ligament stem cells in albino rats : (Record no. 63951)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03224cam a2200337 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223031855.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 171211s2016 ua do f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.09.12.M.Sc.2016.No.C |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Noha Ahmed Elsherif |
| 245 10 - TITLE STATEMENT | |
| Title | Characterization of surface markers of salivary gland stem cells versus periodontal ligament stem cells in albino rats : |
| Remainder of title | In vitro study / |
| Statement of responsibility, etc. | Noha Ahmed Elsherif ; Supervised Ratiba Ghali Abdelmalek , Marwa Sameer Moussa |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | توصيف علامات سطح الخلايا الجذعية للغدد اللعابية مقابل الخلايا الجذعية لرباط اللثة فى الفئران البيضاء : |
| Remainder of title | دراسة معملية |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Noha Ahmed Elsherif , |
| Date of publication, distribution, etc. | 2016 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 97 P. : |
| Other physical details | charts , photographs ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Oral and Dental Medicine - Department of Oral Biology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Objective: The aim of the present study is to isolate and characterize salivary gland stem cells and periodontal stem cells from adult albino rat. A comparison was carried out between their surface markers expression utilizing surface markers for flow cytometry. The odontogenic differentiation of salivary gland stem cells and periodontal ligament stem cells was assessed. Material and methods: Samples were collected from the two sources. PDLSCs and SGSCs were treated with collagenase before centrifugation to allow release of the cells. Cells were cultured in complete culture medium for 12-14 days. After reaching confluence, the isolated cells were characterized by flow cytometry using CD133 and C-Kit. The cells were induced for odontogenic differentiation by placing the cells in odontogenic induction media for 21 days. Odontogenic differentiation was evaluated by Alizarin Red stain and by the expression of dentine sialophosphoprotein (DSPP) which was assessed by RT-PCR. Results: The results showed that successful isolation of stem cells from periodontal ligament and salivary gland was achieved. Cells were successfully sub-cultured and expanded up to passage three. A flow cytometric analysis was done after stem cells isolation. PDLSCs were negatively expressed CD133 and C-Kit while SGSCs showed moderately positive expression of CD133 and C-KIT. The results showed that PDLSCs and SGSCs induced by odontogenic medium were stained by alizarin red at days 14 and 21. RT-PCR results indicated that all cultured cells efficiently differentiate into dentin forming cells and expressed specific DSPP gene which was expressed higher by PDLSCs than SGSCs in days 14 and 21. Conclusion: It can be concluded that PDLSCs show higher differentiation into odontogenic linage than SGSCs |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Albino rats |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Salivary gland stem cells |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Surface markers |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Marwa Sameer Moussa , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ratiba Ghali Abdelmalek , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Samia |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.09.12.M.Sc.2016.No.C | 01010110073693000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.09.12.M.Sc.2016.No.C | 01020110073693000 | 22.09.2023 | CD - Rom | 73693.CD |