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Occurrence of histamine producing bacteria in fish and its public health importance / (Record no. 64742)

MARC details
000 -LEADER
fixed length control field 03345cam a2200349 a 4500
003 - CONTROL NUMBER IDENTIFIER
control field EG-GiCUC
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250223031920.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 180130s2017 ua d f m 000 0 eng d
040 ## - CATALOGING SOURCE
Original cataloging agency EG-GiCUC
Language of cataloging eng
Transcribing agency EG-GiCUC
041 0# - LANGUAGE CODE
Language code of text/sound track or separate title eng
049 ## - LOCAL HOLDINGS (OCLC)
Holding library Deposite
097 ## - Thesis Degree
Thesis Level M.Sc
099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC)
Classification number Cai01.10.18.M.Sc.2017.Ra.O
100 0# - MAIN ENTRY--PERSONAL NAME
Personal name Radwa Ashour Mohamed
245 10 - TITLE STATEMENT
Title Occurrence of histamine producing bacteria in fish and its public health importance /
Statement of responsibility, etc. Radwa Ashour Mohamed ; Supervised Maha Ahmed Sabry , Eman Aly Elghareeb Hamza , Hayam Abdelaal Mansour
246 15 - VARYING FORM OF TITLE
Title proper/short title تواجد الميكروبات المنتجة للهستامين فى الأسماك وأهميتها الصحية
260 ## - PUBLICATION, DISTRIBUTION, ETC.
Place of publication, distribution, etc. Cairo :
Name of publisher, distributor, etc. Radwa Ashour Mohamed ,
Date of publication, distribution, etc. 2017
300 ## - PHYSICAL DESCRIPTION
Extent 109 P. :
Other physical details charts ;
Dimensions 25cm
502 ## - DISSERTATION NOTE
Dissertation note Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Zoonoses
520 ## - SUMMARY, ETC.
Summary, etc. Histamine fish poisoning becomes highly concern not only in public health but also economic aspect. Therefore, this study was attempted to detect histamine producing bacteria (HPB) in fish and fish handlers, as well as in canned tuna. Screening of 207 fish samples using Modified Niven{u2019}s medium (MNM), evidenced 108 isolated colonies were HPB, and significantly higher proportion wasin fresh sardine (95.7%), followed by frozen mackerel (53 %), frozen sardine (36.4 %) and canned tuna (14 %). Among those, only 88 isolates showed HPB based on plating on Violet Red Bile Glucose Agar (VRBG) agar, were 83 isolates identified as Enterobacteriacae, while only 5 isolates were identified as non-Enterobacteriacae. The most common isolates were K. pneumonia, E. aerogenes, Enterobacter cloacae and C. freundii following the biochemical identification using API20E. Out of 83 HP Enterobacteriacea strains from fish muscles 22 isolates (26.5%) were positive for hdc gene using conventional PCR, likewise, Citrobacter braakii evidenced hdc gene. While out of 5 non- Enterobacteriacea strains only one isolate was positive for hdc gene. The measuring of histamine level in some selected positive fish muscles using High-Performance Liquid Chromatography (HPLC) showed that the level of histamine in sardine muscles is significantly higher than that in mackerel muscles. On the other side, out of 20 hand swabs from fish handlers, 19 isolates of HPB were identified as Enterobacteriacea, and classified as K. pneumonia,C. freundii, E. cloacae and K. oxytoca, surprisingly, only one isolate was positive for hdc gene. A strong correlation was noticed between the occurrence of certain species of HP-Enterobacteriaceae in both fish and fish handlers at same localities. Phylogenetic analysis of hdc gene of different bacterial species,highlight the probability that the gram negative hdc gene might get transferred within the same and between the different genus of bacteria, this is alarming as they can be potential hazard to humans
530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE
Additional physical form available note Issued also as CD
653 #4 - INDEX TERM--UNCONTROLLED
Uncontrolled term Canned tuna
653 #4 - INDEX TERM--UNCONTROLLED
Uncontrolled term Mackerel
653 #4 - INDEX TERM--UNCONTROLLED
Uncontrolled term Sardine
700 0# - ADDED ENTRY--PERSONAL NAME
Personal name Eman Aly Elghareeb Hamza ,
Relator term
700 0# - ADDED ENTRY--PERSONAL NAME
Personal name Hayam Abdelaal Mansour ,
Relator term
700 0# - ADDED ENTRY--PERSONAL NAME
Personal name Maha Ahmed Sabry ,
Relator term
856 ## - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a>
905 ## - LOCAL DATA ELEMENT E, LDE (RLIN)
Cataloger Nazla
Reviser Revisor
905 ## - LOCAL DATA ELEMENT E, LDE (RLIN)
Cataloger Shimaa
Reviser Cataloger
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Dewey Decimal Classification
Koha item type Thesis
Holdings
Source of classification or shelving scheme Not for loan Home library Current library Date acquired Full call number Barcode Date last seen Koha item type Copy number
Dewey Decimal Classification   المكتبة المركزبة الجديدة - جامعة القاهرة قاعة الرسائل الجامعية - الدور الاول 11.02.2024 Cai01.10.18.M.Sc.2017.Ra.O 01010110074207000 22.09.2023 Thesis  
Dewey Decimal Classification   المكتبة المركزبة الجديدة - جامعة القاهرة مخـــزن الرســائل الجـــامعية - البدروم 11.02.2024 Cai01.10.18.M.Sc.2017.Ra.O 01020110074207000 22.09.2023 CD - Rom 74207.CD