Comparative evaluation of Ascorbic Acid and Ý-Propiolactone Inactivation efficacy to Vesicular Stomatitis Virus (VSV) as HCV model in Artificially Infected Plasma and Related Protein Profile / (Record no. 65001)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03892cam a2200337 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223031929.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 180214s2017 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.08.06.M.Sc.2017.Ma.C |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Marwa Ibrahim Ali Eltelawy |
| 245 10 - TITLE STATEMENT | |
| Title | Comparative evaluation of Ascorbic Acid and Ý-Propiolactone Inactivation efficacy to Vesicular Stomatitis Virus (VSV) as HCV model in Artificially Infected Plasma and Related Protein Profile / |
| Statement of responsibility, etc. | Marwa Ibrahim Ali Eltelawy ; Supervised Magdy Ali Amin , Aly Fahmy Mohamed |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | تقييم مقارن لكفاءة تثبيط حامض الاسكوربيك ومادة البيتا بروبيولاكتون لفيروس الفزكيولارستوماتيتس كنموذج لفيروس الكبد (ج) فى البلازما المصابة معمليآ و علاقته بشكل البروتين |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Marwa Ibrahim Ali Eltelawy , |
| Date of publication, distribution, etc. | 2017 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 114 Leaves : |
| Other physical details | charts , facsimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Prevalence of contagious agents in whole blood and blood products is a worldwide problem. These products are of great importance to hepatic , hemophilic and autoimmune patients . Safety of blood products is one of the major infection control measures. The present work aimed to evaluate the inactivation potentials of Ýeta propiolactone [BPL] used as 0.0035 M and Ascorbic acid [AA] used as 1.5 / 3 mM activated with 5 Mmol/mm cupper sulphate. Also, monitoring the consequent changes in biological activity of plasma constituents post chemical treatment. Plasma pool from blood bank attendant [BBA] were tested for infectious agents including Hepatitis B , C and Immune Deficiency Syndrome virus (HIV) using PCR and ELISA for Antigen and antibody detection. Infectious viruses free plasma was artificially contaminated with Vesicular Stomatitis virus[VSV]. Artificially infected plasma aliquots were kept at +4oC and 37oC and samples were collected at 15 minutes interval in case of BPL and 1 hr in case of AA treated plasma. Treated plasma was processed for inactivation kinetics using cell culture. Plasma profile was monitored using electrophoresis and coagulation factors were tested using special kits. Our results showed that virucidal activity, data recorded revealed that BPL was significantly faster inactivating agent;[~3hr] [P<0.05] than AA[~24hr] and inactivation depends on thermal condition, time and molarities dependent. Also, Bio-activity of coagulation factors IIIV , IX , PT and aPTT were not significantly influenced [P>0.05] in case of treatment at 37oC than in case of treatment at +4oC, and despite the detected changes of the biological activity, its values were maintained within the normal ranges. Electrophoresis analysis showed that albumin , and G globulin were not influenced significantly post treatment with BPL ,1.5 and 3 mM Ascorbic acid [P>0.05] , accompanied with a moderate depletion rate of Ü1 and Ü2 detected 48 and 72 hr post treatment with Ascorbic acid at 1.5 and 3 mM at +4oC , while a significant change of Ý-globulin was detected post AA 1.5 and 3 mM treatment at 37oC. Finally it can be concluded that BPL and AA can be used as virucidal agents for recycling of infected plasma and maintaining almost the whole bioactivity of plasma constituents |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | HCV |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Related Protein Profile |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Vesicular Stomatitis Virus (VSV) |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Aly Fahmy Mohamed , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Magdy Ali Amin , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Enas |
| Reviser | Cataloger |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.08.06.M.Sc.2017.Ma.C | 01010110074113000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.08.06.M.Sc.2017.Ma.C | 01020110074113000 | 22.09.2023 | CD - Rom | 74113.CD |