Laser induced fluorescence for monitoring bacterial growth / (Record no. 65106)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03641cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223031932.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 180220s2016 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.24.05.M.Sc.2016.Di.L |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Dina Salah Arabi |
| 245 10 - TITLE STATEMENT | |
| Title | Laser induced fluorescence for monitoring bacterial growth / |
| Statement of responsibility, etc. | Dina Salah Arabi ; Supervised Mohamed Abd Elharith Mohamed , Zienab Abd Elfattah Abdelsalam , Hanan Mohamed Abdellatif |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | الوميض المستحث بالليزر لمتابعة النمو البكتيري |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Dina Salah Arabi , |
| Date of publication, distribution, etc. | 2016 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 98 P. : |
| Other physical details | charts , facsimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - National Institute of Laser Enhanced Science - Department of Laser Applications Environmental Metrology Photochemistry and Agriculture |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | The use of CW laser light in two different biological applications (therapeutic and diagnostic) was investigated in this study. Firstly, a 405 nm diode-pumped solid state (DPSS) laser with output power of 100 mW was used as a therapeutic tool against two different species of bacteria in-vitro; Staphylococcus aureus as a gram positive model and Pseudomonas aeruginosa as a gram negative model. The bacterial cell suspensions prepared in 0.9% normal saline were irradiated directly in a 96-well microtitulation plate with 6 different doses (38.2, 76.4, 114.6, 152.8, 191.08, 229.2 J/cm2). Each species showed a different response to the treatment. P. aeruginosa showed a relative sensitivity to the treatment with a linear increasing pattern along with the doses applied. The maximum of 63.2% reduction in cell viability was observed with the maximum dose applied (229 J/cm2). On the other hand, S. aureus showed more resistance to the treatment resulting in a very slow decreasing pattern with the doses. Only 17.6% reduction in the cell viability was recorded as the maximum reduction with the maximum dose applied. Secondly, the laser induced fluorescence (LIF) technique was exploited as a diagnostic tool to simplify detection, and quantification of bacteria based on their auto-fluorescence abilities. Two laser excitation sources (405 nm and 266 nm) with two different bacterial species under investigation (P. aeruginosa and S. aureus) were assessed. The emitted fluorescence spectra were collected and analyzed, and the results came out proving that LIF is a promising technique that can help in discrimination and identification of cells as each sample showed a distinct fluorescence pattern. Also, fluorescence intensity showed an exponential relation with the concentration of the sample (log10 CFU/ml), which may be with further adjustment become helpful in identification of unknown samples. In order to visualize the induced fluorescence inside the cells, a confocal laser scanning microscope was employed revealing the shape of the investigated cells and the fluorescence emission at different wavelengths after excitation with 405 nm laser light which supported the results obtained by LIF |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Fluorescence for monitoring bacterial growth |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Laser induced |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Monitoring bacterial growth |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hanan Mohamed Abdellatif , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mohamed Abdelharith Mohamed , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zienab Abdelfattah Abdelsalam , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Shimaa |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.24.05.M.Sc.2016.Di.L | 01010110074392000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.24.05.M.Sc.2016.Di.L | 01020110074392000 | 22.09.2023 | CD - Rom | 74392.CD |