MARC details
| 000 -LEADER |
|---|
| fixed length control field |
02642cam a2200325 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
EG-GiCUC |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
180310s2016 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
EG-GiCUC |
| Language of cataloging |
eng |
| Transcribing agency |
EG-GiCUC |
| 041 0# - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| 049 ## - LOCAL HOLDINGS (OCLC) |
|---|
| Holding library |
Deposite |
| 097 ## - Thesis Degree |
|---|
| Thesis Level |
Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) |
|---|
| Classification number |
Cai01.12.21.Ph.D.2016.Am.E |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Amal Mahfouz Abdelreheim |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Effective diagnosis of human Toxoplasmosis using superparamagnetic nanoparticles by ELISA technique / |
| Statement of responsibility, etc. |
Amal Mahfouz Abdelreheim ; Supervised Azza Mohamed Elamir , Noha Ahmed Mahana , Alyaa Ahmed Farid |
| 246 15 - VARYING FORM OF TITLE |
|---|
| Title proper/short title |
التشخيص الفعال لمرض التكسوبلازما في الإنسان بإستخدام الجزيئات النانونيه لأشباه المغناطيس الفائقة بتقنية الأليزا |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
|---|
| Place of publication, distribution, etc. |
Cairo : |
| Name of publisher, distributor, etc. |
Amal Mahfouz Abdelreheim , |
| Date of publication, distribution, etc. |
2016 |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
112 P. : |
| Other physical details |
charts , facsimiles ; |
| Dimensions |
25cm |
| 502 ## - DISSERTATION NOTE |
|---|
| Dissertation note |
Thesis (Ph.D.) - Cairo University - Faculty of Science - Department of Zoology |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
Parasitic diseases have a great impact in human health. The gold standard for the diagnosis of the majority of parasitic infections is still conventional microscopy, which presents important limitations in terms of sensitivity and specificity and commonly requires highly trained technicians. More accurate technique, gold nanoparticles (AuNPs) conjugated with anti-Toxoplasma gondii (T. gondii) polyclonal antibody (pAb) was evaluated to increase the sensitivity and specificity of antigen detection assay (sandwich-ELISA and dot-ELISA) for diagnosing human T. gondii infection. In the present study, T. gondii SAG2 antigen was purified (37 KDa) and anti-SAG2 IgG pAb was prepared in rabbits. T. gondii SAG2 antigen was early detected in control and patient sera using nanogold sandwich and nanogold dot-ELISA. Two different nanogold ELISA showed a higher significant sensitivity (94% and 98%) and specificity (96% and 96%) in comparison to normal sandwich and dot-ELISA which showed 92% and 96% sensitivity and 90% and 94% specificity, respectively. From all recorded data and with consideration to sandwich and dot-ELISA assays, nanogold-sandwich and nanogold dot-ELISA assay have a promising and important value as an applicable, fast and accurate diagnostic technique for toxoplasmosis |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE |
|---|
| Additional physical form available note |
Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
Diagnosis |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
Sandwish ELISA |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
T. gondii |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Alyaa Ahmed Farid , |
| Relator term |
|
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Azza Mohamed Elamir , |
| Relator term |
|
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Noha Ahmed Mahana , |
| Relator term |
|
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
|---|
| Cataloger |
Nazla |
| Reviser |
Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
|---|
| Cataloger |
Shimaa |
| Reviser |
Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Koha item type |
Thesis |
