Evaluation of antiviral and anticancer activity of garlic extract (s) through detection of MX and anticancer genes / (Record no. 65478)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03637cam a2200337 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223031944.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 180318s2017 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.08.06.M.Sc.2017.Ra.E |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Rana Hatem Kamal Eldin |
| 245 10 - TITLE STATEMENT | |
| Title | Evaluation of antiviral and anticancer activity of garlic extract (s) through detection of MX and anticancer genes / |
| Statement of responsibility, etc. | Rana Hatem Kamal Eldin ; Supervised Alaa ELdin Mahmoud Shawky , Aly Fahmy Mohamed |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | تقيم النشاط المضاد للأورام والفيروسات لمستخلص الثوم بالكشف عن نشاط جين الإم إكس وبعض الجينات المضادة للأورام فى الزرع النسيجى |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Rana Hatem Kamal Eldin , |
| Date of publication, distribution, etc. | 2017 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 121 P. : |
| Other physical details | charts , facsimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Background: Garlic and allicin (an organosulfur compound of garlic) have been found to possess potent therapeutic effects including anticancer and antiviral potentials. Objective: In vitro evaluation of anticancer and antiviral activity of allicin and aqueous ethanolic garlic extract Method: Cytotoxicity of allicin and garlic extract on normal (Vero & WISH) and cancer (HepG2 & MCF-7) cell lines was measured by MTT assay. Components of aqueous-ethanolic garlic extract were identified using LC-MS/MS and GC-MS analysis. Anticancer activity of allicin and garlic compared to cisplatin were investigated through measuring the expression of BAX, P53 and BCL2 genes using real time RT-PCR. Non-cytotoxic concentration of allicin and garlic were evaluated for their antiviral activity against vesicular stomatitis virus on normal cell lines by end-point method. In addition, the expression of antiviral marker MXA gene was detected by real time RT-PCR. Result: Garlicand allicin reduced the viability of cancer cells in dose dependent manner. The result of real time RT-PCR showed that BAX gene was up-regulated nearly by 3, 3.5 fold (cisplatin), 8.11, 7.2 fold (allicin) and 7, 5.98 fold (garlic), P53 gene was upregulated nearly by 3.83, 4.1 fold (Cisplatin), 7.61, 7.33 fold (allicin) and 6.05, 6.53 fold (garlic) on HepG2 and MCF-7 cell lines, respectively. While BCL2 gene was downregulated nearly by 1.72, 2 fold (cisplatin), 3.33, 4.65 fold (allicin) and 2.82, 3.64 (garlic) on HepG2 and MCF-7 cell lines, respectively. The antiviral marker MXA gene was also upregulated nearly by 6.02, 5.5 fold (allicin), 5.5, 5 fold (garlic) and 1.96, 1.8 fold (interferon) on Vero and on WISH cell lines, respectively. The result of end point method revealed that virus infectivity was reduced in the order of 41%, 43% (allicin), 33%, 28% (garlic) and 63%, 62% (interferon) on Vero and WISH cell lines, respectively. Conclusion: Allicin and garlic extract may induced the apoptosis of liver and breast cancer cell lines through increasing P53 and BAX genes expression and decreasing BCL2 gene expression. In addition, allicin and garlic extract may possess antiviral activity against VSV virus and through increasing the expression of MXA gene |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | BAX |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | BCL2 |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Garlic |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Alaa ELdin Mahmoud Shawky , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Aly Fahmy Mohamed , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Shimaa |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.08.06.M.Sc.2017.Ra.E | 01010110074637000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.08.06.M.Sc.2017.Ra.E | 01020110074637000 | 22.09.2023 | CD - Rom | 74637.CD |