Development of multiplex real time PCR assay for detection of some equine respiratory viruses / (Record no. 65628)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02761cam a2200325 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 180329s2017 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.10.17.M.Sc.2017.Sh.D |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Shimaa Mousleh Sayed Ghoniem |
| 245 10 - TITLE STATEMENT | |
| Title | Development of multiplex real time PCR assay for detection of some equine respiratory viruses / |
| Statement of responsibility, etc. | Shimaa Mousleh Sayed Ghoniem ; Supervised Hussein Aly Hussein Ahmed , Ayman Hany Eldeeb , Mohamed Galal Aggour |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | تطوير أختبار البلمره المتسلسل الحقيقي الوقتي المتعدد للكشف عن بعض الفيروسات التنفسية للفصيلة الخيلية |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Shimaa Mousleh Sayed Ghoniem , |
| Date of publication, distribution, etc. | 2017 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 156 P. : |
| Other physical details | charts , facsimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Virology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Viral respiratory diseases are frequently reported in equine species affecting equine industry and cause huge economic losses. In the present study, we developed a multiplex assay for the simultaneous detection of the main viruses that cause respiratory diseases in equine species, Equine herpesvirus type 1(EHV-1) and 4 (EHV-4) and Equine influenza virus (EIV). The primers and probes amplified only the targeted viruses and there were no inter- assay cross amplifications or non-specific interactions. The multiplex assay efficiencies were 92.5%, 97% and 90% and the monoplex efficiencies were 97.4%, 98.2% and 90.7% for EHV-1, EHV-4 and EIV, respectively. The R square values (Rsq) in both forms were greater than 0.990. The performance of the assay was evaluated by analysing 152 different clinical samples from clinically infected horses. EHV-1 DNA was detected as the single causative agent in 12 samples, EHV-4 DNA in 9 samples and both EHV-1 and EHV-4 were detected in 4 samples. EIV RNA was not detected during this study. The accuracy of the assay was confirmed by comparing these results with those obtained from analysing the same samples using commercial rPCR and rRT- PCR diagnostic kits. This multiplex assay is proven to be a sensitive, specific, accurate and cost- effective method for the detection of the target viruses whether they occur as a single agent or as a part of co-infections |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Equine |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Multiplex real |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Time PCR |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ayman Hany Eldeeb , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hussein Aly Hussein Ahmed , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mohamed Galal Aggour , |
| Relator term | |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Enas |
| Reviser | Cataloger |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.10.17.M.Sc.2017.Sh.D | 01010110074343000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.10.17.M.Sc.2017.Sh.D | 01020110074343000 | 22.09.2023 | CD - Rom | 74343.CD |