Algal-based expression of hemagglutinin (HA2) protein of avian influenza virus (AIV) : (Record no. 65629)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02533cam a2200313 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 180329s2017 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.10.17.M.Sc.2017.Ma.A |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Mahmoud Mohamed Elsayed Bayoumi |
| 245 10 - TITLE STATEMENT | |
| Title | Algal-based expression of hemagglutinin (HA2) protein of avian influenza virus (AIV) : |
| Remainder of title | A step towards a universal vaccine / |
| Statement of responsibility, etc. | Mahmoud Mohamed Elsayed Bayoumi ; Supervised Ausama Abdelraouf A. Yousif , Haitham Mohamed Mohamed Amer |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | التعبير في الطحالب لبروتين التلزن الدموى{u٠٦ئ٢} لفيروس إنفلونزا الطيور إتش٥إن ١ : |
| Remainder of title | خطوة تجاه لقاح شامل |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Mahmoud Mohamed Elsayed Bayoumi , |
| Date of publication, distribution, etc. | 2017 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 189 P. : |
| Other physical details | charts , facsimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Virology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Green biotechnology is the future of biopharmaceuticals production. The use of algae for the production of biopharmaceuticals is of particular interest in vaccine-based control programs. An attempt was made to express avian influenza virus (AIV) immunogens in algae because the virus is a serious economic and public health threat. A commercial eukaryotic expression system was modified to allow expression of AIV H5N1 hemagglutinin subunit 2 (HA2) in the microalga Chlamydomonas reinhardtii (C. reinhardtii). Codon-optimized AIV H5N1 HA2 sequences were synthesized and cloned (coHA2) into a cloning vector pUC-57. coHA2 sequences were then transferred to algal expression vector pChlamy_3/D- TOPO® then to C. reinhardtii strain cc-125. Proper nuclear integration was achieved in 16% of the screened transformants that survived selection in hygromycin-containing TAP media. coHA2 mRNA transcription was confirmed using RT-PCR, GFP expression using Confocal microscopy, AIV HA2 expression was confirmed using western blot analysis utilizing AIV H5N2 chicken antisera. These results warrant further investigation into immunogenic potential of the algae-expressed HA2 |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Avian influenza |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Chlamydomonas reinhardtii |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | HA2 |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ausama Abdelraouf Abdelmoneim Yousif , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Haitham Mohamed Mohamed Amer , |
| Relator term | |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Enas |
| Reviser | Cataloger |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.10.17.M.Sc.2017.Ma.A | 01010110074344000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.10.17.M.Sc.2017.Ma.A | 01020110074344000 | 22.09.2023 | CD - Rom | 74344.CD |