Assessment of some mycobacterial antigens in diagnosis of bovine tuberculosis / (Record no. 66616)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02690cam a2200313 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 180624s2017 ua a f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.10.10.M.Sc.2017.Wa.A |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Wagdy Samir Beshir Youssef |
| 245 10 - TITLE STATEMENT | |
| Title | Assessment of some mycobacterial antigens in diagnosis of bovine tuberculosis / |
| Statement of responsibility, etc. | Wagdy Samir Beshir Youssef ; Supervised Kamelia Mahmoud Osman , Emad Mokhtar Riad |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | تقييم بعض انتيجينات الميكوبكتريا فى تشخيص مرض السل البقرى |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Wagdy Samir Beshir Youssef , |
| Date of publication, distribution, etc. | 2017 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 208 P. : |
| Other physical details | ill ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | In this study, some M. bovis cell extract antigens 2sonicated antigen (SA)3, culture filtrate antigens "short term culture filtrate (ST-CF)" and tuberculin were prepared from 29 mycobacterium isolates after the bacteriological examination of 39 tuberculin positive animals which tested from Elsharkia and Elmenofia governorates by cultural methods. Microscopic examination was conducted for all 39 tuberculous lesions by using Ziehl-Nelseen stain and 27 were positive with a percentage of 69.23%. Real time PCR was carried out on all 29 isolates for identification of recovered colonies and all were identified as Mycobacterium bovis with a percentage of 100%. Extraction of M. bovis with sonication resulted in the recovery of 1.3 mg/ml of soluble protein in the sonicated extract and the protein concentration of M. bovis ST-CF at 2nd week of growth was 2.7 mg/ ml. In addition, the protein concentration of M. bovis prepared tuberculin was 2.0 mg/ml and 1.0 mg/ml of offered PPD. SDS-PAGE was applied for characterization of antigenic extracts and CF of M. bovis and Western blotting analysis was performed. The use of ELISA on serum samples collected from the 1540 cows, before being tested by SICTT, revealed that 131 (8.51%) animals were positive to bTB by using PPD, 139 (9.03%) by using the commercial mixture antigen, 120 (7.79%) by using SA, 125 (8.12%) by using ST-CF and 133 (8.64%) positive animals by using prepared tuberculin, indicating that the use of mixture antigen could help to give more accurate diagnosis |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Antigens |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Bovine tuberculosis |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | ELISA |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Emad Mokhtar Riad , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Kamelia Mahmoud Osman , |
| Relator term | |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Samia |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.10.10.M.Sc.2017.Wa.A | 01010110075247000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.10.10.M.Sc.2017.Wa.A | 01020110075247000 | 22.09.2023 | CD - Rom | 75247.CD |