Microbiological and physiological studies on the production of polysaccharides from local actinomycetes / (Record no. 66629)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02896cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223032018.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 180626s2017 ua do f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.08.06.Ph.D.2017.Ma.M |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Marwa Omar Hussein Elnahas |
| 245 10 - TITLE STATEMENT | |
| Title | Microbiological and physiological studies on the production of polysaccharides from local actinomycetes / |
| Statement of responsibility, etc. | Marwa Omar Hussein Elnahas ; Supervised Magdy Ali Amin , Judy D. Wall , Mohamed Magd Eldin Hussein |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | دراسات ميكروبيولوجية و فيسيولوجية على إنتاج السكريدات العديدة من الأكتنوميستات المحلية |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Marwa Omar Hussein Elnahas , |
| Date of publication, distribution, etc. | 2017 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 197 P. : |
| Other physical details | charts , photographs ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (Ph.D.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Two streptomyces strains were isolated from soil samples and the sequence of ~ 1500 nucleotides of their 16S rDNA showed 99% identity to streptomyces sp. HV10 and 100% identity to streptomyces chattanoogensis respectively. These newly isolated Streptomyces strains produced extracellular polysaccharides (EPS) named MOE6-EPS and MOE7-EPS respectively. The two EPS were characterized by various spectroscopic techniques including fourier transform infrared (FTIR), HPLC, 1HNMR as well as UV visible spectroscopy. MOE6-EPS composed of 71±1% carbohydrate by weight, 3.6±0.1% uronic acid and 0.78±0.1% protein, while MOE7-EPS composed of 98±0.5% carbohydrate by weight, 0.2±0.1% uronic acid and 0.3±0.1% protein. MOE6-EPS and MOE7-EPS showed antioxidant activities by scavenging the hydroxyl free radical scavenging activity and 2,2-diphenyl-2-picryl-hydrazyl (DPPH) radicals. In addition, MOE6-EPS showed high ferrous ion (Fe2+) chelation activity which is another antioxidant activity. Interestingly, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays that were colorimetric assays for NAD(P)H-dependent cellular oxidoreductases and a proxy of the number of viable cells, showed that MOE6-EPS and MOE7-EPS inhibited the proliferation of the human breast cancer cells (MDA-MB-231). Moreover, the scratch wound assay showed that MOE6-EPS and MOE7-EPS reduced the migration of human breast cancer cells (MDA-MB-231) in EPS concentration dependent manner |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Antioxidant activities |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Polysaccharides |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Streptomyces |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Judy D. Wall , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Magdy Ali Amin , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mohamed Magd Eldin Hussein , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Samia |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.08.06.Ph.D.2017.Ma.M | 01010110075259000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.08.06.Ph.D.2017.Ma.M | 01020110075259000 | 22.09.2023 | CD - Rom | 75259.CD |