MARC details
000 -LEADER |
fixed length control field |
02820cam a2200325 a 4500 |
003 - CONTROL NUMBER IDENTIFIER |
control field |
EG-GiCUC |
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
fixed length control field |
180804s2017 ua o f m 000 0 eng d |
040 ## - CATALOGING SOURCE |
Original cataloging agency |
EG-GiCUC |
Language of cataloging |
eng |
Transcribing agency |
EG-GiCUC |
041 0# - LANGUAGE CODE |
Language code of text/sound track or separate title |
eng |
049 ## - LOCAL HOLDINGS (OCLC) |
Holding library |
Deposite |
097 ## - Thesis Degree |
Thesis Level |
Ph.D |
099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) |
Classification number |
Cai01.10.10.Ph.D.2017.We.M |
100 0# - MAIN ENTRY--PERSONAL NAME |
Personal name |
Wessam Youssef Mohamed Ahmed |
245 10 - TITLE STATEMENT |
Title |
Molecular studies on mycobacteria / |
Statement of responsibility, etc. |
Wessam Youssef Mohamed Ahmed ; Supervised Khaled Farouk Mohammed Elamry , Salah Eldin Abdelkarim Selim , Sherif Abdelmonam Omar |
246 15 - VARYING FORM OF TITLE |
Title proper/short title |
دراسات جزيئية على الميكوباكتريا |
260 ## - PUBLICATION, DISTRIBUTION, ETC. |
Place of publication, distribution, etc. |
Cairo : |
Name of publisher, distributor, etc. |
Wessam Youssef Mohamed Ahmed , |
Date of publication, distribution, etc. |
2017 |
300 ## - PHYSICAL DESCRIPTION |
Extent |
164 P. : |
Other physical details |
photographs ; |
Dimensions |
25cm |
502 ## - DISSERTATION NOTE |
Dissertation note |
Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology |
520 ## - SUMMARY, ETC. |
Summary, etc. |
Mycobacterium bovis is the major causative agent of bovine tuberculosis (BTB) and part of the Mycobacterium tuberculosis complex (MTBC). BTB has an impact on the national and international economy, affects the ecosystem via transmission to wildlife and is of public health concern due to its zoonotic potential. Although still present in some industrialized countries, BTB today mostly affects developing countries lacking the resources to apply expensive test and slaughter schemes. Tuberculosis (TB) remains a global health problem despite near eradication in some developed countries. This study was conducted from early winter of 2015 to spring of 2017 to compare between conventional and molecular techniques for detection of mycobacterium bovis (M. bovis) in Egypt. A total of 49 specimens were collected from four major abattoirs (Elbasateen-Elmonieb- Beni-Suef- Alfayoum) to be analyzed bacteriologically and biochemically for: isolation, identification and confirmation of M. bovis with molecular methods. Only 19 isolates were found to be positive slow-growers mycobacterium species by conventional cultivation method on solid medium (LJ medium and Stone brink) and identified biochemically to 17 M. bovis isolates and 2 isolates M. tuberculosis. Genotyping detection of Mycobacterium tuberculosis complex by amplification of ext-RD9 region by real-time PCR was carried out on positive cultures and directly on specimens. Out of 49 DNA templates extracted directly from specimens, 31 specimens were confirmed to be infected by Mycobacterium tuberculosis complex by amplification of ext-RD9 region by real-time PCR |
530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE |
Additional physical form available note |
Issued also as CD |
653 #4 - INDEX TERM--UNCONTROLLED |
Uncontrolled term |
M. bovis |
653 #4 - INDEX TERM--UNCONTROLLED |
Uncontrolled term |
M. tuberculosis |
653 #4 - INDEX TERM--UNCONTROLLED |
Uncontrolled term |
MTBC |
700 0# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Khaled Farouk Mohammed Elamry , |
Relator term |
|
700 0# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Salah Eldin Abdelkarim Selim , |
Relator term |
|
700 0# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Sherif Abdelmonam Omar , |
Relator term |
|
905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
Cataloger |
Nazla |
Reviser |
Revisor |
905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
Cataloger |
Samia |
Reviser |
Cataloger |
942 ## - ADDED ENTRY ELEMENTS (KOHA) |
Source of classification or shelving scheme |
Dewey Decimal Classification |
Koha item type |
Thesis |