Effect of an epigenetic modifier as an anticancer drug on colorectal cancer cell lines / (Record no. 67283)
[ view plain ]
| 000 -LEADER | |
|---|---|
| fixed length control field | 02778cam a2200337 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223032038.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 180903s2018 ua d f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.08.09.M.Sc.2018.Ri.E |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Riham Mohamed Karkeet |
| 245 10 - TITLE STATEMENT | |
| Title | Effect of an epigenetic modifier as an anticancer drug on colorectal cancer cell lines / |
| Statement of responsibility, etc. | Riham Mohamed Karkeet ; Supervised Hanan S. Elabhar , Samia A. Shouman |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | تأثير مغير التخلق المتوالي كمضاد للسرطان في خطوط خلايا سرطان القولون والمستقيم |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Riham Mohamed Karkeet , |
| Date of publication, distribution, etc. | 2018 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 122 P. : |
| Other physical details | charts ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Pharmacology and Toxicology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Cancer is a multifactorial disease and its treatment requires combining different drugs to act synergistically. Epigenetic factors play a key role in cancer etiology and drug response. Modification of these epigenetic changes is a promising avenue for the development of new and effective therapies. 5-Fluorouracil (5-FU), an anti-metabolite, is the gold standard in treatment of colorectal cancer and valproic acid (VPA) is a histone deacetylase inhibitor, thus it has an epigenetic modifying effect. The aim of the current study was to investigate the epigenetic modifying effect of valproic acid (VPA) on 5-florouracil (5-FU) cytotoxic effect in CRC cell lines (Caco2 and HCT 116) and explore the possible underlying mechanism(s) of their interaction. Different combination regimens of 5-FU and VPA were analyzed using the factorial design. The maximum inhibitory combinations, as well as the minimum inhibitory combinations, were chosen to study the differential effect of VPA on various epigenetic markers as global DNA methylation ratio, histone acetyl transferase (HAT) activity and histone 3 (H3) acetylation percentage. Moreover, different metastatic parameters were studied as vascular endothelial growth factor level, matrix metalloproteinases (MMPs) activity and SNAI1 protein expression. Apoptosis was assessed by measuring Caspase-3 level, Caspase-9, ITPK1, AKT and Bcl-2 protein expression. Also, thymidine synthetase (TS) level was determined in cell culture medium |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Anticancer drug |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Colorectal cancer cell lines |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Epigenetic modifier |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hanan S. Elabhar, |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Samia A. Shouman , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Shimaa |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.08.09.M.Sc.2018.Ri.E | 01010110075658000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.08.09.M.Sc.2018.Ri.E | 01020110075658000 | 22.09.2023 | CD - Rom | 75658.CD |