MARC details
000 -LEADER |
fixed length control field |
02864cam a2200325 a 4500 |
003 - CONTROL NUMBER IDENTIFIER |
control field |
EG-GiCUC |
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
fixed length control field |
180912s2017 ua dh f m 000 0 eng d |
040 ## - CATALOGING SOURCE |
Original cataloging agency |
EG-GiCUC |
Language of cataloging |
eng |
Transcribing agency |
EG-GiCUC |
041 0# - LANGUAGE CODE |
Language code of text/sound track or separate title |
eng |
049 ## - LOCAL HOLDINGS (OCLC) |
Holding library |
Deposite |
097 ## - Thesis Degree |
Thesis Level |
M.Sc |
099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) |
Classification number |
Cai01.12.21.M.Sc.2017.Ma.D |
100 0# - MAIN ENTRY--PERSONAL NAME |
Personal name |
Manar Mahmoud Imam Ibrahim |
245 10 - TITLE STATEMENT |
Title |
Diagnosis of acute and chronic schistosomiasis haematobium using purified 26 kDa schistosoma-specific protein / |
Statement of responsibility, etc. |
Manar Mahmoud Imam Ibrahim ; Supervised Azza Mohamed Elamir , Noha Ahmed Mahana , Neamat Amer |
246 15 - VARYING FORM OF TITLE |
Title proper/short title |
تشخيص حالات بلهارسيا المجاري البوليه الحادة والمزمنه بإستخدام البروتين الخاص ( 26 كيلو دالتون) المنقي من ديدان البلهارسيا |
260 ## - PUBLICATION, DISTRIBUTION, ETC. |
Place of publication, distribution, etc. |
Cairo : |
Name of publisher, distributor, etc. |
Manar Mahmoud Imam Ibrahim , |
Date of publication, distribution, etc. |
2017 |
300 ## - PHYSICAL DESCRIPTION |
Extent |
79 P. : |
Other physical details |
charts , facsimiles ; |
Dimensions |
30cm |
502 ## - DISSERTATION NOTE |
Dissertation note |
Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Zoology |
520 ## - SUMMARY, ETC. |
Summary, etc. |
One of the world wide major public health problems is the schistosomiasis that is caused by Schistosoma (S.) heamatobium. It is also one of the main concerns for the public health community in Egypt. There are several immunodiagnostic methods used for that diagnosis of such disease, but some are more sensitive and specific than others. The purified 26 kDa Schistosoma-specific protein (PSPA-26) detection in serum samples is found out to be more valuable in diagnosis; it also helps in the early diagnosis which will lead to the early treatment before the irreversible damage takes place. PSPA-26 was purified from whole worms by DEAESephadex G-75 ion exchange chromatography and then was injected into rabbits to produce specific polyclonal antibodies(anti-PSPA-26 pAb)which is then used as a primary capture in the indirect ELISA technique to reveal its reactivity using infected human sera.The anti-PSPA-26 was then labeled with horse-radish peroxidase (HRP) and used as a secondary capture.Sandwich ELISA was done for serum samples of human and hamsters infected with S. heamatobium.The results revealed a sensitivity of 85% for human and 80% for hamster's samples,and a specificity of 95% for human and 91.1% for hamsters samples by comparing them with those infected with other parasites and control samples. Data obtained concluded that PSPA-26 antigen can be used as a diagnostic marker for S. haematobium infection using the sandwich ELISA which is cost effective and applicable technique |
530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE |
Additional physical form available note |
Issued also as CD |
653 #4 - INDEX TERM--UNCONTROLLED |
Uncontrolled term |
Diagnosis |
653 #4 - INDEX TERM--UNCONTROLLED |
Uncontrolled term |
PSPA-26 |
653 #4 - INDEX TERM--UNCONTROLLED |
Uncontrolled term |
S. haematobium |
700 0# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Azza Mohamed Elamir, |
Relator term |
|
700 0# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Neamat Ame , |
Relator term |
|
700 0# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Noha Ahmed Mahana , |
Relator term |
|
905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
Cataloger |
Nazla |
Reviser |
Revisor |
905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
Cataloger |
Shimaa |
Reviser |
Cataloger |
942 ## - ADDED ENTRY ELEMENTS (KOHA) |
Source of classification or shelving scheme |
Dewey Decimal Classification |
Koha item type |
Thesis |