Study on photoactive nano-hydroxyapatite as a modified photosensitizing agent in cancer cells / (Record no. 67464)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03160cam a2200337 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223032043.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 180915s2017 ua dho f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.24.05.M.Sc.2017.Ah.S |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Ahmed Elsayed Abdelhamid Moustafa |
| 245 10 - TITLE STATEMENT | |
| Title | Study on photoactive nano-hydroxyapatite as a modified photosensitizing agent in cancer cells / |
| Statement of responsibility, etc. | Ahmed Elsayed Abdelhamid Moustafa ; Supervised Souad Ahmed Elfeky , Wafaa Abdallah Ahmed |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | دراسة استخدام جسيمات الهيدروكسى اباتيت النانوية المعدلة و النشطة ضوئيا كوسيط حساس للضوء فى خلايا السرطان |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Ahmed Elsayed Abdelhamid Moustafa , |
| Date of publication, distribution, etc. | 2017 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 96 P. : |
| Other physical details | charts , facsimiles , photographs ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - National Institute for Laser Enhanced Sciences - Department of Laser Applications In Environmental Metrology Photochemistry and Photobiology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Using nanoplatforms as carriers for photosensitizers has been found to enhance the efficacy of photodynamic therapy by improving bioavailability, preventing drug aggregation, and enhancing photosensitizer solubility to obtain a high therapeutic outcome. In this study, the photodynamic cytotoxicity of methylene blue (MB) loaded on hydroxyapatite nanoparticles (HA) was tested against osteosarcoma cancer cell line, Saos-2. HA and HA loaded with methylene blue (HA-NPs-MB) were prepared by chemical precipitation method. The prepared HA and HA-NPs-MB were characterized by TEM, Zeta potential, FTIR, and XRD. TEM images revealed that HA nanoparticles have a rod shape with a diameter around 14 to 17 nm and length around 46 to 64nm. FTIR and zeta potential confirmed the adsorption of cationic MB on HA. XRD pattern was identical to the standard XRD pattern of hydroxyapatite nanoparticles. Incubation of Saos-2 cells for 24 hours with of HA-NPs-MB and then irradiation of cells for 5 minutes with laser emitting diode at 800 nm, causes a reduction of cell viability (determined by MTT assay) more than the free methylene blue. The LC50 was 57.53 æg/ml and 86.99 æg/ml for HA-NPs-MB and free MB, respectively. Thus, nanoformulation of MB greatly reduced the dose of methylene blue required for effective photodynamic therapy. In the absence of laser, no cytotoxicity was observed for either HA-NPs-MB or free MB. No cytotoxicity was detected after incubation of cells with HA in the presence or the absence of laser. This study also investigated the mode of cell death after incubation of cells with free MB and HA-NPs-MB then exposure to laser radiation |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Cancer cells |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Modified photosensitizing agent |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Nano-hydroxyapatite |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Souad Ahmed Elfeky , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Wafaa Abdallah Ahmed , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Samia |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.24.05.M.Sc.2017.Ah.S | 01010110075798000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.24.05.M.Sc.2017.Ah.S | 01020110075798000 | 22.09.2023 | CD - Rom | 75798.CD |