Molecular characterization of BoLA-DRB3 gene and its possible association with serotype O {u2013} FMDV resistance in buffaloes / (Record no. 67640)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03415cam a2200325 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 180924s2018 ua d f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.10.17.M.Sc.2018.Mu.M |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Muhammad Gamal Khodary Ali Omar |
| 245 10 - TITLE STATEMENT | |
| Title | Molecular characterization of BoLA-DRB3 gene and its possible association with serotype O {u2013} FMDV resistance in buffaloes / |
| Statement of responsibility, etc. | Muhammad Gamal Khodary Ali Omar ; Supervised Hussein Ali Hussein Ahmed , Ayman Hanea Eldeeb , Othman Elmahdy Othman |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | في الجاموس O وإحتمالية مصاحبته للمقاومة لفيروس الحمي القلاعية نوع BoLA-DRB3 التوصيف الجزيئي لجين ال |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Muhammad Gamal Khodary Ali Omar , |
| Date of publication, distribution, etc. | 2018 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 105 P. : |
| Other physical details | charts ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Virology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Buffalo is considered as one of the main two sources of good quality meat and milk in Egypt. One of the most economically important viral pathogens of animals is FMD virus, which is highly contagious among cloven hoofed mammals including buffalo. The BoLA-DRB3 functional genes play a significant role in genetic control of immune responses. The present study aimed to investigate the genetic polymorphism of BoLA-DRB3 gene in some Egyptian buffaloes with the assessment of its association with FMD resistance, in addition to investigate the contribution of Foot and Mouth Disease Virus (FMDV) and characterization of the circulating strains between the studied population during 2016.Testing of 40 collected samples of Oropharyngeal, oral epithelial tissue and/or vesicular fluids by real time Reverse transcriptase polymerase chain reaction (rRT-PCR) revealed 33 out of 40 FMDV positive samples.Typing of the highly viral loaded FMDV positive samples by conventional reverse transcriptase polymerase chain reaction (RT-PCR) revealed that all tested samples belonged to serotype O of FMDV. Sequencing and phylogenetic analysis of 5 representative samples clustered the detected viruses with O topotype East Africa-3 (EA-3) viruses. The 302-bp amplified fragments from BoLA-DRB3 exon 2 were digested with HaeIII endonuclease and the results revealed presence of five BoLA-DRB3 genotypes, among them the genotype AA might be associated with FMD-resistance (P<0.05) where its frequency was 88.89% in resistant and 11.1% in susceptible groups. On the other hand, genotype AC could be correlated with susceptibility to FMD in the studied Egyptian buffaloes where it was absent in resistant group. In conclusion, findings of this study suggested that some Egyptian water buffalo might have the ability to relatively resist FMD by mutation which results in changes of the translated amino acids and then the protein structure to perform the regulation of different signaling pathways used by the cell in the long process of choice evolution |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | BoLA-DRB3 |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | FMDV |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Resistance |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ayman Hanea Eldeeb , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hussein Ali Hussein Ahmed , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Othman Elmahdy Othman , |
| Relator term | |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Shimaa |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.10.17.M.Sc.2018.Mu.M | 01010110075939000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.10.17.M.Sc.2018.Mu.M | 01020110075939000 | 22.09.2023 | CD - Rom | 75939.CD |