Isolation and Molecular characterization of Canine Parvovirus from Cairo and Giza in the period of 2014- 2015 / (Record no. 68297)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03367cam a2200325 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 181101s2018 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.10.17.M.Sc.2018.In.I |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Ingy Abdelmegeuid Mohamed Elgendy |
| 245 10 - TITLE STATEMENT | |
| Title | Isolation and Molecular characterization of Canine Parvovirus from Cairo and Giza in the period of 2014- 2015 / |
| Statement of responsibility, etc. | Ingy Abdelmegeuid Mohamed Elgendy ; Supervised Mohamed Abdelhameed Shalaby , Ausama Abdelraouf A. Yousif , Attyat Mohamed Kotb |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | العزل و التصنيف الجزيئي لفيروسات البارفو في الكلاب بمحافظتي القاهرة و الجيزة خلال الفترة من2014- 2015 |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Ingy Abdelmegeuid Mohamed Elgendy , |
| Date of publication, distribution, etc. | 2018 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 151 P. : |
| Other physical details | charts , facsimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Virology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Dogs are the most popular companion animal in Egypt. They could be affected dramatically by many diseases resulted in huge money losses especially among high breeds. Canine parvovirus 2 (CPV-2) is one of the most important viral enteric pathogen for both domestic and wild canids causing severe hemorrhagic enteritis all over the world since 1978. Due to the continuous evolution of the CPV-2, new antigenic variants always emerge causing increase infection in different ages and species. By time and with the emergence of new antigenic variants, this might lead to incidence of vaccination failure cases. Therefore, the efficacy of the current used CPV vaccine against these new variants has to be under further examination to follow up the virus continuous evolution. In the present study, 50 fecal swabs were collected from diseased unvaccinated dogs suffering from gastrointestinal disorders during a period from 2014 to 2015, from Cairo and Giza in Egypt. The collected samples were tested by immunochromatographic diagnostic kit, only 8 out of 50 samples were positive. The 8 positive samples were isolated and propagated in different cell lines (BHK-21, MDCK, and Vero cells). Only 6 out of 8 samples were positive and gave CPE in cell lines. The 6 positive samples were identified by fluorescent antibody technique and all were positive. The 6 samples were introduced to conventional PCR where the 6 samples gave positive bands in the gel electrophoresis. Only 2 positive canine parvovirus PCR products with the local field vaccinal strain (VSVRI vaccine) were sent for sequencing and comparing between them. According to the Genebank, the two samples were 100% homologous and 98% query coverage with each others. When compared to the local vaccinal strain, each sample was 99% homologous and 99% query coverage. Our data suggest that there is no need to change the local field vaccine (VSVRI). The two samples are closely related to CPV2a genotype |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Canine Parvovirus-2 (CPV-2) |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | CPV-2a |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | PCR |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Attyat Mohamed Kotb , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ausama Abdelraouf A. Yousif , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mohamed Abdelhameed Shalaby , |
| Relator term | |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Enas |
| Reviser | Cataloger |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.10.17.M.Sc.2018.In.I | 01010110076338000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.10.17.M.Sc.2018.In.I | 01020110076338000 | 22.09.2023 | CD - Rom | 76338.CD |