MARC details
| 000 -LEADER |
|---|
| fixed length control field |
02896cam a2200313 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
EG-GiCUC |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
181104s2018 ua d f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
EG-GiCUC |
| Language of cataloging |
eng |
| Transcribing agency |
EG-GiCUC |
| 041 0# - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| 049 ## - LOCAL HOLDINGS (OCLC) |
|---|
| Holding library |
Deposite |
| 097 ## - Thesis Degree |
|---|
| Thesis Level |
Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) |
|---|
| Classification number |
Cai01.10.10.Ph.D.2018.He.C |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Heba Farouk Abdelaziz |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Comparative study between serological and molecular diagnosis of Mycoplasma / |
| Statement of responsibility, etc. |
Heba Farouk Abdelaziz ; Supervised Mona Ibrahim Hassan Elenbaawy , Soad Abdelaziz Abdelwanes |
| 246 15 - VARYING FORM OF TITLE |
|---|
| Title proper/short title |
دراسة مقارنة بين التشخيص السيرولوجى و الجزيئي للميكوبلازما |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
|---|
| Place of publication, distribution, etc. |
Cairo : |
| Name of publisher, distributor, etc. |
Heba Farouk Abdelaziz , |
| Date of publication, distribution, etc. |
2018 |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
133 P. : |
| Other physical details |
charts ; |
| Dimensions |
25cm |
| 502 ## - DISSERTATION NOTE |
|---|
| Dissertation note |
Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
Mycoplasmosis is one of the most important diseases in poultry that causes severe economic losses and high rate of mortality among the different poultry species. The causative agent of this disease is Mycoplasma spp.; the most circulating species in Egypt are Mycoplasma synoviae and Mycoplasma gallisepticum which are detected in different poultry sectors and populations. This presented study aims to facilitate the detection and differentiation between these two species of mycoplasma by rapid and sensitive multiplex real time PCR using specific sets of primers and probes for each species. A total of 200 samples were gathered for this study from different poultry populations as 100 broiler farms, 50 layer farms and 50 samples of wild birds, the geographical distribution was considered during the sample collection trying to study the situation of Mycoplasma spp incidence in whole Egypt. Therefore, the results show that 47 farms were positive for Mycoplasma gallisepticum (31.3%); 12 out of 100 broiler farms (12%) and 35 out of 50 layer farms (70%) were positive for Mycoplasma gallisepticum. While, Mycoplasma synoviae was positive in 38 out of 150 farms ( 25%), 1 out of 100 in broilers (1%) and 37 out of 50 in layers (74%). By contrast, all the 50 samples of wild birds were negative for both MG, MS by Multiplex real time PCR. From 150 pooled serum samples tested from different breeds and governorates for MG and MS by SPA and ELISA test .68 (45.3%) were positive for MG and 56 (37.3%) for MS by SPA, while ELISA 43(28.7%) were positive for MG and 56(37.3%).Weak statistical correlation was observed between all serological tests (SPA and ELISA), and they should only be used for initial screening |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE |
|---|
| Additional physical form available note |
Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
Multiplex PCR |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
Mycoplasma |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
Wild bird |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Mona Ibrahim Hassan Elenbaawy , |
| Relator term |
|
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Soad Abdelaziz Abdelwanes , |
| Relator term |
|
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
|---|
| Cataloger |
Enas |
| Reviser |
Cataloger |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
|---|
| Cataloger |
Nazla |
| Reviser |
Revisor |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Koha item type |
Thesis |
