MARC details
| 000 -LEADER |
|---|
| fixed length control field |
02678cam a2200325 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
EG-GiCUC |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
181104s2018 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
EG-GiCUC |
| Language of cataloging |
eng |
| Transcribing agency |
EG-GiCUC |
| 041 0# - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| 049 ## - LOCAL HOLDINGS (OCLC) |
|---|
| Holding library |
Deposite |
| 097 ## - Thesis Degree |
|---|
| Thesis Level |
Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) |
|---|
| Classification number |
Cai01.08.06.Ph.D.2018.Am.A |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Amany Maged Ibrahim |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Analysis study on the dimerization requirements of the Staphylococcus aureus type VII secretion system components EsxB and EsxD / |
| Statement of responsibility, etc. |
Amany Maged Ibrahim ; Supervised Mohammed Abdelhalim Ramadan , Yasser M. Ragab , Khaled Ahmed Aly |
| 246 15 - VARYING FORM OF TITLE |
|---|
| Title proper/short title |
فى البكتيريا المكورة العنقودية الذهبية EsxB و EsxD دراسة تحليلية على متطلبات التفاعل لمكونات النظام السابع المفرز ل |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
|---|
| Place of publication, distribution, etc. |
Cairo : |
| Name of publisher, distributor, etc. |
Amany Maged Ibrahim , |
| Date of publication, distribution, etc. |
2018 |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
169 P. : |
| Other physical details |
charts , facsimiles ; |
| Dimensions |
25cm |
| 502 ## - DISSERTATION NOTE |
|---|
| Dissertation note |
Thesis (Ph.D.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology |
| 520 ## - SUMMARY, ETC. |
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| Summary, etc. |
Type VII secretion system (T7SS) plays a vital role in bacterial virulence, survival and persistence in tissue hosts. It has been identified in several gram positive bacteria including Staphylococcus aureus and was reported to be involved in persistence of abscess in deep host tissues. Deleting any of the 11 genes comprising staphylococcal T7SS, diminished the abscess in mice renal abscess model. EsxB and EsxD are two T7SS secretory proteins that are known to strongly interact. Using error-prone random PCR mutagenesis coupled with bacterial two-hybrid screening, amino acid number 53; Asparagine, was identified as the interaction domain for EsxB. On the other hand, N-terminal truncations from EsxD followed by bacterial two-hybrid analysis, identified amino acids number 11 to 15 to be involved in dimerization of EsxD with EsxB. Furthermore, copper was found to be crucial for WT EsxB:EsxD interaction, where the interaction was abolished when E.coli DHM1 cells harboring WT EsxB:EsxD were grown in the presence of the specific copper chelator; Ammonium tetra thiomolybdate. Analysis of the dynamics of protein interactions in T7SS is a gateway to discovering novel approaches to neutralize bacterial pathogenesis and virulence, in turn diminishing its effects on host cells |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE |
|---|
| Additional physical form available note |
Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
EsxB |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
T7SS |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
Type VII secretion system |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Khaled Ahmed Aly , |
| Relator term |
|
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Mohammed Abdelhalim Ramadan , |
| Relator term |
|
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Yasser Elmohammady Ragab , |
| Relator term |
|
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
|---|
| Cataloger |
Enas |
| Reviser |
Cataloger |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
|---|
| Cataloger |
Nazla |
| Reviser |
Revisor |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Koha item type |
Thesis |
