Important virulence factors and related genes in uropathogenic E. coli and their relation to fluoroquinolone resistance / (Record no. 69192)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02993cam a2200337 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223032136.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 181222s2018 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.11.19.Ph.D.2018.Ha.I |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Hanaa Fathy Aly Abdelatty |
| 245 10 - TITLE STATEMENT | |
| Title | Important virulence factors and related genes in uropathogenic E. coli and their relation to fluoroquinolone resistance / |
| Statement of responsibility, etc. | Hanaa Fathy Aly Abdelatty ; Supervised Magda Ibrahim Mohamed Ayoub , Noha Mahmoud Abdelrahim Gohar |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | عوامل الضراوة المهمة وجيناتها فى العصيات القولونية المسببة لعدوى المجارى البولية وعلاقتها بمقاومة المضاد الحيوى فلوروكينولون |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Hanaa Fathy Aly Abdelatty , |
| Date of publication, distribution, etc. | 2018 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 118 P. : |
| Other physical details | charts , facsimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Microbiology and Immunology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Escherichia coli(E. coli) is the predominant pathogen causing urinary tract infection. E. coli expresses a wide spectrum of virulence factors that enable it to cause the disease. The present study was conducted on 50 E. coli isolates to assess some important phenotypic virulence factors of E. coli: haemolytic activity, cytotoxic activity and haemagglutination and their relations to the following virulence genes: hlyA, cnf-1 and papC, by using multiplex PCR. Fluoroquinolones susceptibility was done using disk diffusion method.The results showed that 30% of the isolates were Ý- haemolytic, 18% of the isolates showed haemagglutination with human RBCs, with higher prevalence for MRHA (12%) than MSHA (6%), and 92% of the isolates showed morphological changes, detachment and lysis in HEp-2 cells. Multiplex PCR showed that the most prevalent gene was cnf-1(30%), while 26% of isolates were positive for hlyA and 26% of isolates were positive for papC. Only one UPEC isolate showed the simultaneous presence of papC, cnf-1 and hlyA while 10 isolates were harbouring hlyA and cnf-1, 2 isolates were harbouring cnf-1 and papC genes and 2 isolates were harbouring hlyA and papC simultaneously. We found that hlyA gene was significantly detected in the Ý- haemolytic than non- haemolytic isolates. As regard fluoroquinolones susceptibility, 60% of the isolates were resistant to the used fluoroquinolones with no significant difference between resistant and susceptible isolates as regard virulence factors and genes |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Escherichia coli |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Multiplex PCR |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Virulence |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Magda Ibrahim Mohamed Ayoub , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Noha Mahmoud Abdelrahim Gohar , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Shimaa |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.11.19.Ph.D.2018.Ha.I | 01010110076567000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.11.19.Ph.D.2018.Ha.I | 01020110076567000 | 22.09.2023 | CD - Rom | 76567.CD |