MARC details
000 -LEADER |
fixed length control field |
02729cam a2200325 a 4500 |
003 - CONTROL NUMBER IDENTIFIER |
control field |
EG-GiCUC |
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
fixed length control field |
190615s2019 ua dho f m 000 0 eng d |
040 ## - CATALOGING SOURCE |
Original cataloging agency |
EG-GiCUC |
Language of cataloging |
eng |
Transcribing agency |
EG-GiCUC |
041 0# - LANGUAGE CODE |
Language code of text/sound track or separate title |
eng |
049 ## - LOCAL HOLDINGS (OCLC) |
Holding library |
Deposite |
097 ## - Thesis Degree |
Thesis Level |
M.Sc |
099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) |
Classification number |
Cai01.10.17.M.Sc.2019.Yo.P |
100 0# - MAIN ENTRY--PERSONAL NAME |
Personal name |
Youssef Abdallah Elsayed AbdElhafiz |
245 10 - TITLE STATEMENT |
Title |
Preparation of inactivated lumpy skin disease virus vaccine using ISA 206 and ISA 50 adjuvants / |
Statement of responsibility, etc. |
Youssef Abdallah Elsayed AbdElhafiz ; Supervised Mohammed Abdelhameed Shalaby , Ayman Hany M. Eldeeb , Mervat Mohamed Ali |
246 15 - VARYING FORM OF TITLE |
Title proper/short title |
تحضير لقاح مثبط لفيروس مرض الجلد العقدى بإستخدام المحسنات المناعية إيزا 50 وإيزا206 |
260 ## - PUBLICATION, DISTRIBUTION, ETC. |
Place of publication, distribution, etc. |
Cairo : |
Name of publisher, distributor, etc. |
Youssef Abdallah Elsayed AbdElhafiz , |
Date of publication, distribution, etc. |
2019 |
300 ## - PHYSICAL DESCRIPTION |
Extent |
73 P. : |
Other physical details |
charts , facsimiles , photoghraphs ; |
Dimensions |
25cm |
502 ## - DISSERTATION NOTE |
Dissertation note |
Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Virology |
520 ## - SUMMARY, ETC. |
Summary, etc. |
Lumpy skin disease (LSD) is one of most serious livestok diseases in Egypt since 1988 when the disease spread widely all over the country. since that attenuated sheep pox virus (SPV) used for vaccination cattle flocks against LSD due to immunological relationship between LSD and SPV. There have been numerous attempts to produce live attenuated LSD vaccine but it has stopped due to the seriousness of the disease's return to its virulence and the post vaccinal reaction of the vaccine so we prepare inactivated LSD virus(LSDV) vaccine to immunize the cattle with safe vaccine .Ismailia isolate of LSD was propagated and titrated on Madin Darby Bovine Kidney Cells (MDBK) till reach the highest titer (10 ⁵·⁵ TCID₅₀/ml). The inactivation process was performed using Bromoethylamine hydro bromide (BEI) that did not affect the immunogenic characters of the LSDV and the process last for 18 hours with 1% concentration of BEI at 37{u00B0}c. The inactivated LSDV was tested on MDBK cells and the animals free of antibodies for residual live virus and determination of minimal protective dose (MPD) of prepared inactivated LSDV. The MPD was 1.5 ml of the inactivated virus and the vaccine was prepared by adding equal volume of the adjuvants (Montanide ISA 50 and Montanide ISA 206) to the total quantity 3ml of the vaccine |
530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE |
Additional physical form available note |
Issued also as CD |
653 #4 - INDEX TERM--UNCONTROLLED |
Uncontrolled term |
Inactivated virus vaccine |
653 #4 - INDEX TERM--UNCONTROLLED |
Uncontrolled term |
ISA 206 |
653 #4 - INDEX TERM--UNCONTROLLED |
Uncontrolled term |
Lumpy skin disease |
700 0# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Ayman Hany M. Eldeeb , |
Relator term |
|
700 0# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Mervat Mohamed Ali , |
Relator term |
|
700 0# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Mohammed Abdelhameed Shalaby , |
Relator term |
|
905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
Cataloger |
Nazla |
Reviser |
Revisor |
905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
Cataloger |
Shimaa |
Reviser |
Cataloger |
942 ## - ADDED ENTRY ELEMENTS (KOHA) |
Source of classification or shelving scheme |
Dewey Decimal Classification |
Koha item type |
Thesis |