Synthesis of novel hybrid steroids with possible cytotoxicity and pro-apoptotic effects on human hepatocellular carcinoma in vitro / (Record no. 73263)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02592cam a2200337 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223032340.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 190804s2018 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.12.25.M.Sc.2018.Ma.S |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Manar Ahmed Ahmed Elhinnawi |
| 245 10 - TITLE STATEMENT | |
| Title | Synthesis of novel hybrid steroids with possible cytotoxicity and pro-apoptotic effects on human hepatocellular carcinoma in vitro / |
| Statement of responsibility, etc. | Manar Ahmed Ahmed Elhinnawi ; Supervised Rafaat Milad Mohareb , Gamal Abdelmegeed Abdelghany |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | تشييد مركبات أسترويدية جديدة و إحتمالية تأثيرها كمضادات لتكاثر الخلايا و محفزات علي الموت المبرمج لخلايا سرطان الكبد خارج جسم الكائن الحي |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Manar Ahmed Ahmed Elhinnawi , |
| Date of publication, distribution, etc. | 2018 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 94 P. : |
| Other physical details | charts , facsimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Biotechemistry |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | A series of pregnenolone derivatives were synthesized and evaluated for anti-cancer activity against hepatocellular carcinoma cell line (HepG2). The synthesized steroidal derivatives 3, 4, 5, 6, 7, 8a and 8b were assessed for their cytotoxic activities using MTT (3-(4,5-Dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide) assay. Apoptotic activity was evaluated using dual acridine orange/ethidium bromide staining method and DNA fragmentation assay. The expression level of the pro-apoptotic genes Bak and Bax as well as anti-apoptotic genes Bcl-xL and Bcl-2 were analyzed using quantitative real time PCR. The results showed that compounds 4 and 6 exhibited cytotoxic activity (IC50s, 36.97 ± 2.18 and 18.46 ± 0.64 oM, respectively), whereas compounds 5 and 7 displayed weak cytotoxic activity (IC50s, 93.87 ± 8.30 oM and 93.48 ± 4.14 oM, respectively). All synthesized pregnenolone derivatives induced apoptosis through DNA fragmentation. Compounds 4 and 6 increased early and late apoptotic cell percentages while compounds 3, 5, 7 and 8b increased either early or late apoptotic cell percentage |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Cytotoxicity |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Novel hybrid steroids |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Pro-apoptotic |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Gamal Abdelmegeed Abdelghany , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Rafaat Milad Mohareb , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Shimaa |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.12.25.M.Sc.2018.Ma.S | 01010110078872000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.12.25.M.Sc.2018.Ma.S | 01020110078872000 | 22.09.2023 | CD - Rom | 78872.CD |