Comparative Study Using MALDI TOF MS Biotyper, Recent Molecular Assays and Conventional Method for Detecting Specified Microbial Contaminants in topical preparations / (Record no. 73785)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03085cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223032355.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 190910s2019 ua do f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.08.06.M.Sc.2019.Sa.C |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Salma Ashraf Mohammed Elqadee |
| 245 10 - TITLE STATEMENT | |
| Title | Comparative Study Using MALDI TOF MS Biotyper, Recent Molecular Assays and Conventional Method for Detecting Specified Microbial Contaminants in topical preparations / |
| Statement of responsibility, etc. | Salma Ashraf Mohammed Elqadee ; Supervised Mohammad Abdalhaleem Ramadan , Omneya Mohammed Helmy , Omneya Mohammed Helmy , Tariq H. Almorsy |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | دراسة للمقارنة بين استخدام تقنية الإمتصاص الضوئي و الفحوصات الجزيئية الحديثة و الطرق التقليدية للكشف عن البكتيريا المُسبّبة لتلوث المستحضرات الصيدلية الجلدية |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Salma Ashraf Mohammed Elqadee , |
| Date of publication, distribution, etc. | 2019 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 77 P. : |
| Other physical details | charts , photographs ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Absence of pathogenic bacteria is a must for passing the microbiological quality control inspection of non-sterile pharmaceutical products. The identification of microbial contaminants by conventional methods is time-consuming and labor-intensive. The overall aim of the study was to test non-conventional methods as polymerase chain reaction (PCR) and Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometery Biotyper (MALDI-TOF) for rapid identification of Pseudomonas aeruginosa and Staphylococcus aureus in topical pharmaceutical preparations. Four different topical preparations and three raw materials were artificially inoculated with tested microorganisms, at an initial cell count of 100 CFU/ml, and were overnight pre-enriched in Tryptic soy broth (TSB). MALDI-TOF was tested for identification of target microorganisms using different sample preparation methods; the direct transfer method using single separated colony of target microorganism and two other methods using the incubated broth directly; Formic acid extraction method and newly proposed method , Direct pellet method. PCR targeting oprL (Pseudomonas aeruginosa) and mRNA nuclease gene (Staphylococcus aureus) was also tested using two DNA extraction methods; Mild lysis method was used for detection of single microbial contaminant, Uniplex PCR while QIAmp DNA minikit was used for detection of both microorganisms, duplex and uniplex PCR |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | MALDI-TOF |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Non-sterile |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | PCR |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mohammad Abdalhaleem Ramadan , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Omneya Mohammed Helmy , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Tariq H. Almorsy , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Asmaa |
| Reviser | Cataloger |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.08.06.M.Sc.2019.Sa.C | 01010110079010000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.08.06.M.Sc.2019.Sa.C | 01020110079010000 | 22.09.2023 | CD - Rom | 79010.CD |