Molecular recognition of brucella based on matrix-assisted laser desorption ionization time-of-flight mass spectrometry and molecular imprinted polymers / (Record no. 73859)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03131cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223032357.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 190914s2019 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.10.10.M.Sc.2019.Ab.M |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Abdoulrazak Omar Ali |
| 245 10 - TITLE STATEMENT | |
| Title | Molecular recognition of brucella based on matrix-assisted laser desorption ionization time-of-flight mass spectrometry and molecular imprinted polymers / |
| Statement of responsibility, etc. | Abdoulrazak Omar Ali ; Supervised Wagih A. Gad Elsaid , Mahmoud D. Elhariri , Ashraf E. M. Sayour |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | تعرف جزيئي على البروسيلا قائم على مطياف الكتلة المعتمد على زمن طيران الببتيدات المتأينة بالليزر الممتز بمساعدة وسط مح{u٠٦أأ}ط والمبلمرات المطبوعة جزيئيا |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Abdoulrazak Omar Ali , |
| Date of publication, distribution, etc. | 2019 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 120 P. : |
| Other physical details | charts , facsimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Brucella is an expanding genus of Gram-negative intracellular wide host ranging pathogens. This work aimed at investigating molecular recognition of Brucella by MALDI-TOF MS proteomic fingerprinting as well as novel plastic antibodies by developing and characterizing molecularly-imprinted hydrogels to grasp all surface epitopes at one go for whole cell recognition of Brucellaabortus, B. melitensisand B. suis known to exist among livestock in Egypt. An MSP library of 11 reference Brucella strains was created. A dendrogram for reference strains was plotted to analyze phyloproteomic relations. Based on bacteriologic and proteomic biotyping of 45 field isolates, a map revealed the geographic distribution of Brucellamelitensis and B. abortus from 69 unvaccinated seropositive ruminants in 12 governorates during 2015.The MALDI-TOF MS was re-evaluated as a revolutionary molecular tool for Brucella identification reviewing the pros and cons of the technique suggesting recent methods to tackle existing hitches. E{uFB00}ective bacterial recognition using a cell-imprinted polymer (CIP) formed on a 96-well microplate was achieved within 30 minutes.The polymer could discriminate the target strain from other strains with high selectivity reaching approximately 20 folds. It was concluded that bacteriologic and MALDI results fully matched thanks to the limited diversity of Brucella isolates and the narrow MSP library. The CIP approach proved valuable for rapid direct Brucella recognition and quantification colorimetrically in microtiter plates after full validation |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Brucella |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Hydrogel |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | MIPS |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ashraf E. M. Sayour , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mahmoud D. Elhariri , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Wagih A. Gad Elsaid , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Shimaa |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.10.10.M.Sc.2019.Ab.M | 01010110079290000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.10.10.M.Sc.2019.Ab.M | 01020110079290000 | 22.09.2023 | CD - Rom | 79290.CD |