Nanoparticles based approach for direct detection of urinary miRNA in diabetic nephropathy / (Record no. 75283)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02530cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223032441.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 191118s2019 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.08.01.M.Sc.2019.Su.N |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Suzy Mahmoud Mohamed Morsy |
| 245 10 - TITLE STATEMENT | |
| Title | Nanoparticles based approach for direct detection of urinary miRNA in diabetic nephropathy / |
| Statement of responsibility, etc. | Suzy Mahmoud Mohamed Morsy ; Supervised Manal Fouad Ismail , Sanaa Eissa Mohamed , Naglaa Mohamed Elsayed |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | ٳستخدام جزيئات الذهب النانومتريه للكشف عن الحمض النووى الريبوزى المتناهى الصغر فى الأعتلال الكلوى لمرضى البول السكرى |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Suzy Mahmoud Mohamed Morsy , |
| Date of publication, distribution, etc. | 2019 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 132 P. : |
| Other physical details | charts , facsimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Biochemistry |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | miRNA-34a was reported as urinary marker for early detection of diabetic nephropathy. In this work, a simple colorimetric gold nanoparticle (AuNP) assay was developed for rapid and sensitive detection of unamplified urinary exosomal miRNA-34a based on the surface plasmon resonance (SPR) property of AuNPs. The nano-assay uses citrate-capped AuNPs, specific probe targets miRNA-34a and NaCl as an aggregation inducer. miRNA-34a-probe hybrid stabilizes AuNPs against salt induced aggregation. The method performed in totally extracted urinary exosomal microRNA from normal and type 2 diabetic subjects. The AuNP assay results were compared with conventional qRT-PCR results.The sensitivity and specificity for qualitative detection of urinary miRNA-34a using the AuNP assay were 81.08% and 69.6% respectively.The sensitivity of detection of urinary miRNA-34a by qRT-PCR was (83.8%), while specificity was (93.3%).The method is simple, cost-efficient, has a short turnaround time and requires minimal equipment and personnel for urinary miRNA-34a detection |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Diabetes mellitus |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Diabetic complications |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Diabetic Nephropathy |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Manal Fouad Ismail , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Naglaa Mohamed Elsayed , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sanaa Eissa Mohamed , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Shimaa |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.08.01.M.Sc.2019.Su.N | 01010110080037000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.08.01.M.Sc.2019.Su.N | 01020110080037000 | 22.09.2023 | CD - Rom | 80037.CD |