MARC details
| 000 -LEADER |
|---|
| fixed length control field |
02718cam a2200325 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
EG-GiCUC |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
191119s2019 ua o f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
EG-GiCUC |
| Language of cataloging |
eng |
| Transcribing agency |
EG-GiCUC |
| 041 0# - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| 049 ## - LOCAL HOLDINGS (OCLC) |
|---|
| Holding library |
Deposite |
| 097 ## - Thesis Degree |
|---|
| Thesis Level |
M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) |
|---|
| Classification number |
Cai01.07.01.M.Sc.2019.We.I |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Wessam Mohamed Ali Rslan |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Improvement of sugar beet using some new biotechnological techniques / |
| Statement of responsibility, etc. |
Wessam Mohamed Ali Rslan ; Supervised Mohamed Ibrahim Masr , Naglaa Ahmed Abd Allah , Roba Medhat Ismail |
| 246 15 - VARYING FORM OF TITLE |
|---|
| Title proper/short title |
تحسين بنجر السكر باستخدام بعض التقنيات البيوتكنولوجيه الحديثه |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
|---|
| Place of publication, distribution, etc. |
Cairo : |
| Name of publisher, distributor, etc. |
Wessam Mohamed Ali Rslan , |
| Date of publication, distribution, etc. |
2019 |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
67 P. : |
| Other physical details |
photographs ; |
| Dimensions |
25cm |
| 502 ## - DISSERTATION NOTE |
|---|
| Dissertation note |
Thesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Agronomy |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
This work was carried out to get synthetic seed and herbicide resistant sugar beet genotypes, using micro-shoot tips encapsulated in 4% (w/v) sodium alginate with 100 mM Ca (NO3) 24H2O as a complex solution. Two solutions were evaluated for their encapsulated vitality efficiency; SA1 solution (MS, 3% sucrose, 4% sodium alginate, 2% sorbitol and 2% mannitol) and SA2 solution (MS, 3% sucrose, 1.3 BAP+4% sodium alginate, 2% sorbitol and 2% mannitol). Synthetic seeds were kept in the dark at 40C to slow down all the tissues growth and stored for different time ranging from 1week to 8 weeks. For getting herbicide resistant plants, agrobacterium carrying the plant vector PISV 2678 was used for transformation of shoot tips of El-Magary and Farida cultivars. PCR and leaf painting test were used to select transgenic plants. Results showed that SA2 was more efficient than SA1 in encapsulated re-growth. Prolonging storage up to 8 weeks reduced significantly germination percentage. Elmagary cultivar had higher transformation efficiency (18%) than farida (12%). Spraying transgenic sugar beet plants with basta had no significant effect on growth traits, but significantly reduced sucrose percentage. Genetic stability of encapsulated plants that was carried out using RAPD PCR analysis showed no significant variability between encapsulated and control plants. Applying this technique can be recommended for developing synthetic seeds as cost effective and time saving method |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE |
|---|
| Additional physical form available note |
Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
Artificial seeds |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
Storage ability |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
Sugar beet |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Mohamed Ibrahim Masri , |
| Relator term |
|
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Naglaa Ahmed Abdallah , |
| Relator term |
|
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Roba Medhat Ismail , |
| Relator term |
|
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
|---|
| Cataloger |
Nazla |
| Reviser |
Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
|---|
| Cataloger |
Samia |
| Reviser |
Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Koha item type |
Thesis |
