Effect of diclofenac sodium on the expression levels of adhesion-related genes (asa1 and efaA) and the EfrAB efflux pump genes in enterococcal clinical isolates / (Record no. 75686)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03094cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223032454.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 191207s2019 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.11.19.Ph.D.2019.Ab.E |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Abeer Khaled Mokhtar Abuelazayem |
| 245 10 - TITLE STATEMENT | |
| Title | Effect of diclofenac sodium on the expression levels of adhesion-related genes (asa1 and efaA) and the EfrAB efflux pump genes in enterococcal clinical isolates / |
| Statement of responsibility, etc. | Abeer Khaled Mokhtar Abuelazayem ; Supervised Heba Hamed Arnaout , Marwa Salah Mostafa , Maha Mahmoud Kotb |
| 246 1# - VARYING FORM OF TITLE | |
| Title proper/short title | فى المكورات المعوية السريرية EfrAB وجينات مضخات efaA و asa1 تأثير ديكلوفيناك الصوديوم على مستويات التعبير عن الجينات المرتبطة بالالتصاق |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Abeer Khaled Mokhtar Abuelazayem , |
| Date of publication, distribution, etc. | 2019 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 117 P. : |
| Other physical details | charts , facimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Microbiology and Immunology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Antimicrobial drug resistance has become an emerging threat and a matter of concern worldwide. Many steps are being taken to reduce this problem, like gradually limiting antibiotics administration against pathogenic bacteria and searching for non-antibiotic compounds that have antibacterial activity through different mechanisms. The aim of this work was to evaluate the antibacterial effect of diclofenac sodium on enterococcal growth, ciprofloxacin resistance and biofilm forming ability. The effect of exposure of enterococcal isolates to diclofenac sodium on their expression of efflux pump genes (efrA and efrB) and adherence-related genes (asa1 and efaA) was also investigated. Fifty Enterococcal isolates were retrieved from 36 urine and 14 pus specimens. Antimicrobial activity of diclofenac and ciprofloxacin was determined by agar dilution method.Their effect on biofilm formation was determined by tissue culture plate method. The expression of asa1, efaA, efrA and efrB genes was determined by RT-PCR before and after exposure to diclofenac. Ciprofloxacin resistance was reported in 54% of the enterococcal isolates. Diclofenac was found to have antibacterial activity, as it inhibited the growth of enterococcal isolates at MIC of 400-800æg/ml. Although diclofenac induced a statistically significant reduction in the expression of efflux pump genes (efrA and efrB) (P < 0.001), it failed to reduce either ciprofloxacin resistance rate or ciprofloxacin MICs. Biofilm formation was reported in 64% of the isolates |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Ciprofloxacin |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Diclofenac |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Enterococci |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Heba Hamed Arnaout , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Maha Mahmoud Kotb , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Marwa Salah Mostafa , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Shimaa |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.11.19.Ph.D.2019.Ab.E | 01010110080251000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.11.19.Ph.D.2019.Ab.E | 01020110080251000 | 22.09.2023 | CD - Rom | 80251.CD |