The anti-oxidant and apoptotic effect of pomegranate extract on head and neck squamous cell carcinoma cell line : (Record no. 76402)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03305cam a2200337 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223032516.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 200120s2018 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.09.14.Ph.D.2018.Na.A |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Nada Nour Eldin Abdelaziz Negm |
| 245 14 - TITLE STATEMENT | |
| Title | The anti-oxidant and apoptotic effect of pomegranate extract on head and neck squamous cell carcinoma cell line : |
| Remainder of title | In vitro study / |
| Statement of responsibility, etc. | Nada Nour Eldin Abdelaziz Negm ; Supervised Louloua Mohamed Fathy , Safa Fathy Abdelghany |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | تأثير خلاصة الرمان كمضاد للأكسدة و محفز للموت المبرمج لسرطان الخلايا القشرية فى الرأس و الرقبة : |
| Remainder of title | دراسه فى المختبر |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Nada Nour Eldin Abdelaziz Negm , |
| Date of publication, distribution, etc. | 2018 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 117 P. : |
| Other physical details | charts , facsimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (Ph.D.) - Cairo University - Faculty of Oral and Dental Medicine - Department of Oral and Maxillofacial Pathology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Head and neck cancers were ranked as the sixth most common cancer worldwide and still a major cause for death. Objectives: the aim of the study was to examine the antioxidant and apoptotic effect of pomegranate extract (PE), reactive oxygen species (ROS), and their combination on oral squamous cell carcinoma (OSCC) cell line. Methods and results: Two cell lines were used, namely, SCC15 derived from tongue squamous cell carcinoma and normal gingival keratinocytes, which was used as normal control. They were divided into eight groups, four for each cell line, three of which were subjected to PE, H2O2 and a combination of both with the fourth being used as control. Doses were determined using the MTT viability assay. Cell cycle and apoptosis analysis were assessed using flow cytometry. Early and late apoptosis indicators, namely cytochrome c (CYT-C) and caspase3, respectively were measured by Elisa and the antioxidant capacity was detected by Nrf2 at both RNA and transcribed protein levels using RT-PCR and Elisa, respectively. Results revealed that PE, H2O2 and their combination have promising effects. A selective cytotoxic and apoptotic inducing effect were seen towards cancer cells rather than normal cells. The percentage of viable cells were decreased in the treated SCC groups compared to normal groups and untreated control groups in G0/G1 and S cell cycle phases. On the contrary, increase in mean percentage of cells in G2/M and apoptosis phases in all SCC treated groups was observed. The levels of CYT-C and caspase3, were elevated in the treated SCC groups compared to normal cell groups and untreated control groups. Moreover, upregulation of the Nrf2 at both gene and protein levels was also detected, which reflected the antioxidant effect of pomegranate and the defense-inducing mechanism of H2O2 |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Head and neck |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Pomegranate extract |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Squamous cell carcinoma |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Louloua Mohamed Fathy , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Safa Fathy Abdelghany , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Asmaa |
| Reviser | Cataloger |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.09.14.Ph.D.2018.Na.A | 01010110080674000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.09.14.Ph.D.2018.Na.A | 01020110080674000 | 22.09.2023 | CD - Rom | 80674.CD |