Quantitative measurements of cell free DNA and mutation associated in lung cancer / (Record no. 76641)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02717cam a2200337 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223032523.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 200212s2020 ua d f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.12.25.M.Sc.2020.Es.Q |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Esraa Bassam Taha Abdelsalam |
| 245 10 - TITLE STATEMENT | |
| Title | Quantitative measurements of cell free DNA and mutation associated in lung cancer / |
| Statement of responsibility, etc. | Esraa Bassam Taha Abdelsalam ; Supervised Ahmed Helmy Mahmoud Elwahy , Mohamed Sedky Elsaid Mossa |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | القياس الكمى للحمض النووى المتحرر من الخلية و الطفره المرتبطة بسرطان الرئة |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Esraa Bassam Taha Abdelsalam , |
| Date of publication, distribution, etc. | 2020 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 136 P. : |
| Other physical details | charts ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Biotechnology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | The concept of using liquid biopsies in identification of genetic tumor profiling has been researched as a new potential noninvasive technique in cancer detection. Elevated levels of circulating cell free DNA (cf DNA) in plasma has been reported as promising biomarker in diagnosis of cancer. The aim of this study was to investigate if cf DNA marker can distinguish lung cancer patients and healthy control and to study if cf DNA have a role in tumor response to chemotherapy with EGFR-TKI treatment in lung cancer. Additionally, it was suggested that smoking affect the integrity of DNA and by using three markers ALU 115, ALU 247 and DNA integrity index, the degree of effect on apoptosis and necrosis could be examined. Finally, we compared HRM (High Resolution Melting) analysis and sequencing for testing mutation in exon 19 EGFR that have a predictive role for various therapies in NSCLC. Methods: Plasma samples were obtained from 50 lung cancer patients, 25 from them receiving chemotherapy and 50 healthy controls, 25 from them are heavy smoker were chosen for this study. Cf DNA was extracted using Triton/heat/ phenol (THP) method and quantification was measured by q-PCR using ALU 115 and ALU 247, their diagnostic and prognostic potential was estimated using receiver operating characteristics (ROC). Lung cancer patients and heavy smoker were tested for EGFR exon 19 mutation by HRM and Sanger sequencing |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Apoptosis |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | DNA integrity |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | EGFR-TKI |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ahmed Helmy Mahmoud Elwahy , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mohamed Sedky Elsaid Mossa , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Asmaa |
| Reviser | Cataloger |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.12.25.M.Sc.2020.Es.Q | 01010110080791000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.12.25.M.Sc.2020.Es.Q | 01020110080791000 | 22.09.2023 | CD - Rom | 80791.CD |