MARC details
| 000 -LEADER |
|---|
| fixed length control field |
03112cam a2200325 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
EG-GiCUC |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
200223s2020 ua o f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
EG-GiCUC |
| Language of cataloging |
eng |
| Transcribing agency |
EG-GiCUC |
| 041 0# - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| 049 ## - LOCAL HOLDINGS (OCLC) |
|---|
| Holding library |
Deposite |
| 097 ## - Thesis Degree |
|---|
| Thesis Level |
Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) |
|---|
| Classification number |
Cai01.10.10.Ph.D.2020.Mo.D |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Moataz Mohamed Magdy Hessin |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Development of immunochromatographic lateral flow test for rapid detection of clostridium perfringens Ü, Ý and f toxins in clinical samples / |
| Statement of responsibility, etc. |
Moataz Mohamed Magdy Hessin ; Supervised Rafik Tawfik Soliman , Ahmed Samir Mohamed , Yasser Ahmed Abdalla |
| 246 15 - VARYING FORM OF TITLE |
|---|
| Title proper/short title |
تطوير طريقة الانتشار الجانبى المناعية للكشف السريع عن سموم بكتيرياالكلوستيريديم برفرنجينس من نوع الفا و بيتا وابسلون فى العينات الاكلينيكية |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
|---|
| Place of publication, distribution, etc. |
Cairo : |
| Name of publisher, distributor, etc. |
Moataz Mohamed Magdy Hessin , |
| Date of publication, distribution, etc. |
2020 |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
132 P. : |
| Other physical details |
photographs ; |
| Dimensions |
25cm |
| 502 ## - DISSERTATION NOTE |
|---|
| Dissertation note |
Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
Clostridium perfringens toxins is ranked as one of the most common causes of food borne infections and is of major concern as public health problems worldwide for human and animals. The increase in the incidence of foodborne diseases that is often associated with outbreaks, threaten global public health security and raise international concern. Therefore, in the present study a lateral flow immunochromatographic kits have been developed for rapid detection of C. perfringens toxins types, alpha (Ü), beta (Ý) and epsilon (f) in clinical samples. C. perfringens toxins have been prepared, purified and inactivated with 0.2% formalin. Polyclonal antibodies specific to C. perfringens toxins types Ü, Ý and f toxoids have been prepared in rabbits and guinea pigs. The toxin- specific polyclonal antibodies prepared in rabbits were labeled with gold chloride nanoparticles. The prepared toxin specific rabbit and guinea pigs antibodies and goat anti-rabbit antibodies have been utilized in development of lateral flow immunochromatographic test (LFT) that have been evaluated for detection of C. perfringens Ü, Ý and f toxins in clinical samples. The sensitivity, specificity and accuracy of the developed LFT test have been determined by comparison with a commercially available ELISA kits used for detection of these toxins. The prepared LFT was capable to detect C. perfringens Ü, Ý and f toxins in quantities of 2ug/ml, 250ng/ml and 60ng/ml, respectively. 100 poultry suspected fecal samples was examined both with the prepared LFT and ELISA kits for C. perfringens Ü, Ý and f toxins to reveal the validity of developed LFT |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE |
|---|
| Additional physical form available note |
Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
C. perfringens |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
LFT |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
Ü, Ý and f toxins |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Ahmed Samir Mohamed , |
| Relator term |
|
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Rafik Tawfik Soliman , |
| Relator term |
|
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Yasser Ahmed Abdalla , |
| Relator term |
|
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
|---|
| Cataloger |
Nazla |
| Reviser |
Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
|---|
| Cataloger |
Samia |
| Reviser |
Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Koha item type |
Thesis |
