Regeneration and transformation using glutaredoxin-2 gene on grapevine rootstocks / (Record no. 76835)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03351cam a2200325 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 200302s2020 ua dho f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.07.10.Ph.D.2020.Ah.R |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Ahmed Ahmed Mohammed Barakat |
| 245 10 - TITLE STATEMENT | |
| Title | Regeneration and transformation using glutaredoxin-2 gene on grapevine rootstocks / |
| Statement of responsibility, etc. | Ahmed Ahmed Mohammed Barakat ; Supervised Mohamed Hassanen Soliman , Basita Abbas Hussein , Nahla Abdelfattah Awad |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | فى أصول العنبGlutaredoxin-2استيلاد والتحول الوراثى باستخدام جين |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Ahmed Ahmed Mohammed Barakat , |
| Date of publication, distribution, etc. | 2020 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 202 P. : |
| Other physical details | charts , facsimiles , photographs ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (Ph.D.) - Cairo University - Faculty of Agriculture - Department of Genetics |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | This study was carried out to establish reliable techniques to be used for introducing GRX-2 gene into the two grapevine rootstocks (SO4 and Freedom). This approach was performed using Agrobacterium tumefaciens during the period from 2015 to 2019 at the Biotechnology Research Lab. and Plant Tissue Culture Lab. Horticulture Research Institute, Agricultural Research Center, Egypt. The aim of this work was to optimize an efficient transformation system for of the two rootstocks involving the GRX-2 gene by using Agrobacterium tumefaciens mediated technique. This gene donates recipient plant salinity tolerance and subjecting two rootstocks transgenic plants to salinity stress to evaluate their stress tolerance. Genetic transformation of an elite white poplar genotype SO4 and Freedom rootstocks was performed with the vector pRI 101-ON DNA in Agrobacterium tumefaciens strain LBA4404 containing the Glutaredoxin-2 gene in order to enhance the resistance to abiotic stress. The time period of axillary buds infection with Agrobacterium tumefaciens suspension culture (0, 10, 20 and 25 min with different cnocentration of kanamycin in {u00BE}MS media) was tested for the transformation frequency. The highest frequency of transformation was obtained with axillary bud explants infected with Agrobacterium culture for 10 minutes at the concentration of OD ₆₀₀= 0.8 with Kanamycin 100mg/l. Molecular analysis using PCR proved the presence and integration of the transgenes in the genome of the transgenic plants. Field experiments were conducted to investigate the effect of the introduced GRX-2 gene on two rootstocks at salt concentrations (0, 2000, 2500 and 3000 ppm). The data showed that the transgenic lines expressed different levels of salt tolerance as expressed by the performance of vegetative characters (plants plant height, number of leaves, root length and stem diameter), pigments contents (chlorophyll A, B and carotenoids), total Phenol, proline content and enzymes activity. These results show that the GRX-2 gene enhance salt tolerance character |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Agrobacterium |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Rootstocks |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | SO4 |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Basita Abbas Hussein , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mohamed Hassanen Soliman , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Nahla Abdelfattah Awad , |
| Relator term | |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Asmaa |
| Reviser | Cataloger |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.07.10.Ph.D.2020.Ah.R | 01010110080950000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.07.10.Ph.D.2020.Ah.R | 01020110080950000 | 22.09.2023 | CD - Rom | 80950.CD |