Effect of Amygdalin and Milk thistle extract on HepG2 cell line / (Record no. 77404)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02977cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223032548.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 200826s2020 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.12.02.Ph.D.2020.Ka.E |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Karima Mahmoud Nasr Aldin |
| 245 10 - TITLE STATEMENT | |
| Title | Effect of Amygdalin and Milk thistle extract on HepG2 cell line / |
| Statement of responsibility, etc. | Karima Mahmoud Nasraldin ; Supervised Abdelagawad Ali Fahmi , Mohamed Ali Eldesouky , Ibrahim Yehia Abdelkader |
| 246 1# - VARYING FORM OF TITLE | |
| Title proper/short title | تأثير الأميجدالين ومستخلص الحرشف البرى على خط خلايا سرطان الكبد |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Karima Mahmoud Nasr Aldin , |
| Date of publication, distribution, etc. | 2020 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 149 P. : |
| Other physical details | charts , facsmilies ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (Ph.D.) - Cairo University - Faculty of Science - Department of Biochemistry |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | The purpose of this study was to examine the effect of Amygdalin with and without zinc on HepG2 cell line ,to study the possible mechanism of action of amygdalin as a natural anti-cancer agent and to study the effect of natural milk thistle seed extract and the commercial silymarin drug (Legalon) on HepG2 cell line. The MTT method was used to evaluate the cytotoxicity of Amygdalin without zinc, Amygdalin with 20omol Zinc, Amygdalin with 800omol Zinc, M.T extract and Silymarin(Legalon) on HepG2 cell lines. Furthermore, the cell cycle distribution assay was determined by flow cytometry.Apoptosis was confirmed by Annexin V/PI double staining. Moreover, we evaluated the mean level of cytochrome c oxidase, P53, Bcl2, Bax, cytochrome c and caspase 3 level in all treated and untreated HepG2 cells. Amygdalin without zinc showed anti-HepG2 activity. Furthermore ,HepG2 cell line treatment with Amygdalin with 20omol Zinc and Amygdalin with 800omol Zinc showed a highly significant apoptotic effect than the apoptotic effect of Amygdalin without zinc. The cell cycle arrst at G2/M increased by increasing the concentration of zinc and M.T extract showed more cell cycle arrest at G2/M than Legalon. The percentage of early and late apoptosis increased by increasing the zinc concentration while Milk thistle showed more early and late apoptosis than legalon.All treated HepG2 cells showed significant increase in the mean levels of P53,Bax, cytochrome c and caspase 3 . while it decreased the mean level of Bcl2 and cytrochrome c oxidase. Both M.T extract and Silymarin showed a significant apoptotic effect on HepG2 via mitochondrion-dependent pathway |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Amygdalin |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Laetrile |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Vitamin B17 |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Abdelagawad Ali Fahmi , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ibrahim Yehia Abdelkader , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mohamed Ali El Desouky , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Amira |
| Reviser | Cataloger |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.12.02.Ph.D.2020.Ka.E | 01010110081264000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.12.02.Ph.D.2020.Ka.E | 01020110081264000 | 22.09.2023 | CD - Rom | 81264.CD |