Biotechnological detection of some marker genes in cancer cell and their relation to natural anticancer compounds / (Record no. 77676)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03149cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223032556.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 200910s2020 ua d f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.07.04.M.Sc.2020.Mu.B |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Mustafa Tareq Moawad |
| 245 10 - TITLE STATEMENT | |
| Title | Biotechnological detection of some marker genes in cancer cell and their relation to natural anticancer compounds / |
| Statement of responsibility, etc. | Mustafa Tareq Moawad ; Supervised Faten Mohamed Abouelella , Eman Ahmed Hanafy , Neemat Mohamed Kassem |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | الكشف عن بعض الجينات كدلالات للسرطان بالتنقية الحيوية و علاقتها بمضادات السرطان الطبيعية |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Mustafa Tareq Moawad , |
| Date of publication, distribution, etc. | 2020 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 110 P. : |
| Other physical details | charts ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Agricultural Biochemistry |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Leukemia is a rapidly progressive disease which is one of the most common causes of death worldwide.Several therapy modalities have been employed in the treatment of leukemia such as natural anticancer compounds which found in plants e.g. Saussurea Lappa (S. lappa). Cytotoxic activity of S. lappa extracts was determined in relation to CCAAT/Enhancer Binding Protein Alpha (C/EBPA) gene expression, in addition to phytochemical analysis and antioxidant activity. Successive extraction of S. lappa root powder using methanol, ethanol, acetone, ethyl acetate and chloroform solvents was done; the twelve fractions were separated from chloroform extract by thin layer chromatography (TLC). Estimation of total phenolic and total flavonoid was done together with antioxidant. Cytotoxic activity of S. lappa extracts and twelve fractions which have been tested on three leukemia cell cultures (CML, AML, ALL) by MTT assay. C/EBPA gene expression was detected by quantitative real time PCR (qRT-PCR).The chloroform extract of S.lappa showed the highest cytotoxic activity against three cancer cell cultures. Regarding C/EBPA gene expression in leukemic cells after addition of chloroform fractions (C2+C9) from the S. lappa plant, a statistically significant difference (P value 0.008) with (C9) compound was found this denotes the higher cytotoxic activity of (C9) on the leukemia cells. The S. lappa roots contain phenol and flavonoid compounds, which have a high cytotoxic effect on leukemic cells and improve C/EBPA gene expression which is a known good prognostic marker. Further studies of S.lappa for the elucidation of the cytotoxic mechanisms considered as immense importance to deal with leukemia must be done in thefuture |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Antioxidant |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Phytochemical analysis |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Saussurea lappa |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Eman Ahmed Hanafy , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Faten Mohamed Abouelella , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Neemat Mohamed Kassem , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Amira |
| Reviser | Cataloger |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.07.04.M.Sc.2020.Mu.B | 01010110081499000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.07.04.M.Sc.2020.Mu.B | 01020110081499000 | 22.09.2023 | CD - Rom | 81499.CD |