Development of lateral flow immunochromatographic kits for rapid detection of Pasteurellamultocida and Mannheimiahemolyticafrom cattle suffering respiratory infections / (Record no. 78096)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03103cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223032609.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 201006s2019 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.10.10.M.Sc.2019.Ma.D |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Mai Anwar Abdelsamea |
| 245 10 - TITLE STATEMENT | |
| Title | Development of lateral flow immunochromatographic kits for rapid detection of Pasteurellamultocida and Mannheimiahemolyticafrom cattle suffering respiratory infections / |
| Statement of responsibility, etc. | Mai Anwar Abdelsamea ; Supervised Rafik Tawfik Soliman , Rehab Mohamed Elhelw , Rafik Hamed Sayed |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | تطوير اختبار التدفق الجانبى المناعى للكشف عن بكتيريا الباستريلا مالتوسيدا والمانهيميا هيمولتيكا فى الماشيه المصابة بعدوى الجهاز التنفسى |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Mai Anwar Abdelsamea , |
| Date of publication, distribution, etc. | 2019 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 68 P. : |
| Other physical details | charts , facsimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Bovine respiratory disease is a major cause of economic losses in dairy beef and feedlot cattle due to increase mortality rate, intensive cost of treatment and prevention in addition to reduction of carcass value. In the present work it was planned to improve the control ofthese infections through acceleration of the field identificationof some of the important pathogens causing these infections.This workwas planned fordevelopment of simple rapid field test. The developed test was designed for identification of two members of the Pasteurellaceae family, Mannheimiahaemolytica (formerly Pasteurellahaemolytica biotype A) and Pasteurellamultocida, these two speciesare frequently associated withBRD.In the present work a fully identified pure culture ofP.multocida and M. hemolytica was obtained and a pathogen specific antigens was prepared.P.multocida and M.hemolytica specific polyclonal antibodies were prepared raised in rabbits and guinea pig.The pathogen specific IgG was purified by caprylic acid method and the titer of this pathogen specific IgG wasdetermined using ELISA.The pathogens specific rabbit polyclonal IgG antibodies were conjugated withcolloidal gold nanoparticles and used for preparation of lateral flow kits for detection of P.multocida and M. hemolytica.The sensitivity,specificity and accuracy of the developed kits were determined using bacteriological examination as astandard gold test It was88.2%,92.4% and 91% respectively For P.multocida and forM.hemolytica80.6%,94.2% and 90% respectively |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Lateral flow immune diffussion kit |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | M.hemolytia |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | P.multocida |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Rafik Hamed Sayed , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Rafik Tawfik Soliman , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Rehab Mohamed Elhelw , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Shimaa |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.10.10.M.Sc.2019.Ma.D | 01010110081692000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.10.10.M.Sc.2019.Ma.D | 01020110081692000 | 22.09.2023 | CD - Rom | 81692.CD |