Studying lysin B from mycobacteriophages as potential antimycobacterial / (Record no. 78548)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02990cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223032623.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 201103s2020 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.08.06.Ph.D.2020.Ah.S |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Ahmed Hassan Korany Ramadan |
| 245 10 - TITLE STATEMENT | |
| Title | Studying lysin B from mycobacteriophages as potential antimycobacterial / |
| Statement of responsibility, etc. | Ahmed Hassan Korany Ramadan ; Supervised Magdy A. Amin , Tamer M. Essam , Tarek Dishisha |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | من لاقمات البكتيريا المتفطرة كمضادات محتملة للبكتيريا المتفطرة Iysin Bدراسة |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Ahmed Hassan Korany Ramadan , |
| Date of publication, distribution, etc. | 2020 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 230 P . : |
| Other physical details | charts , facsmilies ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (Ph.D.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Mycobacteriophages possess different sets of lytic enzymes for disruption of the complex cell envelope of the mycobacteria host cells and release of the viral progeny. Therefore, mycobacteriophage endolysins have emerged as a potential alternative to the current antimycobacterial agents. This study focuses on Lysin B (LysB) enzymes, mycolylarabinogalactan esterases, which cleave the ester bond between the arabinogalactan and mycolic acids in the mycolylarabinogalactan{u2013} peptidoglycan (mAGP) complex in the cell envelope of mycobacteria. A comparative bioinformatics study was performed using multiple in silico techniques e.g. Multiple sequence alignment and structural analysis studies and showed that LysB-D29, the only enzyme with solved three-dimensional structure, shares several common features with esterases (lacking lid domain) and lipases (acting on long chain lipids). Additionally, sequence and structural comparisons of 30 LysB homology models showed great variation in domain organizations and total protein length with major differences in the loop-5 motif harboring the catalytic histidine residue. Docking of different p-nitrophenyl ligands (C4-C18) to LysB-3D models revealed that the differences in length and residues of loop-5 contributed towards wide diversity of active site conformations (long tunnels, deep and superficial funnels, shallow bowls and narrow buried cave) resembling that of lipases, cutinases and esterases. Three LysB enzymes (LysB-D29, -SWU1 and - BabyRay) were recombinantly produced and characterized with respect to their enzymatic and antibacterial activities |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Antimycobacterial |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Multiple sequence alignment |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Mycolylarabinogalactan esterases |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Magdy A. Amin , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Tamer M. Essam , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Tarek Dishisha , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Amira |
| Reviser | Cataloger |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.08.06.Ph.D.2020.Ah.S | 01010110081925000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.08.06.Ph.D.2020.Ah.S | 01020110081925000 | 22.09.2023 | CD - Rom | 81925.CD |