MARC details
| 000 -LEADER |
|---|
| fixed length control field |
03251cam a2200325 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
EG-GiCUC |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
201130s2020 ua d f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
EG-GiCUC |
| Language of cataloging |
eng |
| Transcribing agency |
EG-GiCUC |
| 041 0# - LANGUAGE CODE |
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| Language code of text/sound track or separate title |
eng |
| 049 ## - LOCAL HOLDINGS (OCLC) |
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| Holding library |
Deposite |
| 097 ## - Thesis Degree |
|---|
| Thesis Level |
M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) |
|---|
| Classification number |
Cai01.08.06.M.Sc.2020.Na.S |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Nazly Reda Elsayed Moaty |
| 245 12 - TITLE STATEMENT |
|---|
| Title |
A study on the potential in vitro activity of natural products on biofilm formation by clinical and environmental isolates of pseudomonas spp. / |
| Statement of responsibility, etc. |
Nazly Reda Elsayed Moaty ; Supervised Mohammed Abdelhaleem Ramadan , Lina Jamil Abdelhafez , Reham Samir Mohammed |
| 246 15 - VARYING FORM OF TITLE |
|---|
| Title proper/short title |
دراسة معملية على المواد الطبيعية على تثبيط تكوين الغشاء الحيوى بواسطة عزلات من السودموناس سريرية و بيئية |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
|---|
| Place of publication, distribution, etc. |
Cairo : |
| Name of publisher, distributor, etc. |
Nazly Reda Elsayed Moaty , |
| Date of publication, distribution, etc. |
2020 |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
121 P. : |
| Other physical details |
charts ; |
| Dimensions |
25cm |
| 502 ## - DISSERTATION NOTE |
|---|
| Dissertation note |
Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
Multidrug-resistant opportunistic pathogen Pseudomonas aeruginosa produces surface-associated communities called biofilm, which protect pathogens by forming a complex permeability barrier for antibiotics. Biofilm formation contributes to persistence and chronic infection caused by Pseudomonas aeruginosa. In this study, we investigated the potential activity of Camellia sinensis (green tea), Olea europaea (olive), Styrax benzoin, Ocimum basilicum, Humulus lupulus, Ruta graveolans, and propolis extracts on biofilm formation, pyocyanin production and twitching motility of P. aeruginosa isolates. Moreover, the effect of green tea and olive leaf extracts were investigated on the transcription of some biofilm related genes. A total of 204 isolates of Pseudomonas spp. collected from Egyptian hospitals. Susceptibility test of the recovered isolates was done using disc diffusion method. The capability of biofilm formation by isolates was evaluated by the Congo red and 96 well polystyrene Microtiter plate methods. The minimum inhibitory concentration (MIC) of these herbal extracts investigated using the broth microdilution method. Sub-MIC of herbal extracts mixed with strong biofilm former-MDR Pseudomonas isolates to determine the percentage of biofilm inhibition. The expression of lasI, lasR, rhlI and rhlR genes in isolated strains was detected using real-time rt-PCR. Susceptibility test revealing that about 49% of the isolates were multidrug-resistant.More than 90% of the isolates were biofilmforming, 26% were strong biofilm producers, 39% were moderate biofilm producers, 25% were weak biofilm producers and 10% couldn{u2019}t form any detectable biofilm. Sub-inhibitory concentrations of plant extracts mixed with strong biofilm former- MDR Pseudomonas isolates to determine the percentage of biofilm inhibition |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE |
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| Additional physical form available note |
Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED |
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| Uncontrolled term |
Biofilm |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
Natural products |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
Pseudomonas |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Lina Jamil Abdelhafez , |
| Relator term |
|
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Mohammed Abdelhaleem Ramadan , |
| Relator term |
|
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Reham Samir Mohammed , |
| Relator term |
|
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
|---|
| Cataloger |
Nazla |
| Reviser |
Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
|---|
| Cataloger |
Shimaa |
| Reviser |
Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Koha item type |
Thesis |
