The Effect of SERCA2 transfected stem cells on induced wound in type1 diabetes rat model compared to stem cells / (Record no. 79121)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03551cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223032641.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 201206s2020 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.11.16.M.Sc.2020.Ah.E |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Ahlam Mohamed Gharieb |
| 245 14 - TITLE STATEMENT | |
| Title | The Effect of SERCA2 transfected stem cells on induced wound in type1 diabetes rat model compared to stem cells / |
| Statement of responsibility, etc. | Ahlam Mohamed Gharieb ; Supervised Maha Baligh Zickri , Zeinab Ahmed Altayeb , Hala Ahmed Elsherif |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | تاثير تعداد الخلايا الجذعية بجين سيركا2 مقارنة بالخلايا الجذعية على الجرح المحدث فى نموذج الجرذ للنوع الاول السكرى |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Ahlam Mohamed Gharieb , |
| Date of publication, distribution, etc. | 2020 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 158 P . : |
| Other physical details | charts , facsmilies ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Histology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | The present study aimed at investigating the therapeutic effect of SERCA2 gene transfected adipose mesenchymal stem cells (AMSCs) on induced wound in type1 diabetes mellitus (T1DM) in male albino rat model and comparing the effect to that of nontransfected AMSCs. Forty two adult male albino rats were divided into: Donor Group included 3 rats for stem cell isolation, culture, phenotyping, labeling and SERCA2 preparation. Group Ї included 9 rats. Group II (Diabetic Wounded Group) included 10 rats. Diabetes was induced by a single intraperitoneal (IP) injection of STZ at a dose of 50 mg/kg. The rats were exposed to wound induction (1x1cm skin incision) on the middle part of the back the day following confirmation of diabetes. Group III (Diabetic Wounded and Non-transfected AMSCs treated Group) included 10 rats. 1x106 of cultured and PKH26 labeled rat cells were injected IP. Group IV (Diabetic Wounded and SERCA2 transfected AMSCs treated Group) included 10 rats. 1x106 of cultured, green fluorescent protein (GFP) labeled and SERCA2 1.5 microgram (og) lipofectamine transfected were injected IP. The rats were sacrificed 3 weeks following the day of confirmation of diabetes. Blood glucose level, skin Ca2+ concentration, western blot analysis, quantitative polymerase chain reaction (qPCR), histological, immunohistochemical, fluorescent microscopic, morphometric and statistical studies were done The mean value of blood glucose indicated a significant increase in the diabetic group compared to the control and the treated groups. The mean Ca ion concentration and mean SERCA2 protein values indicated a significant decrease in the diabetic group compared to all other groups and in AMSCs group versus control and SERCA2 group. Group II demonstrated wide separation of the wound edges, detached parts of the epidermis, disrupted dermal fibers, detached sebaceous glands, follicles lined by 1 layer of cells, multiple microfollicles. Thickened and thinned wound edges, multiple vacuolated keratinocytes, decreased or loss of granular content of granular cell layer,disorganized areas and vacuoles were seen |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Diabetes mellitus |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Skin |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Wound |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hala Ahmed Elsherif , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Maha Baligh Zickri , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zeinab Ahmed AlTayeb , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Amira |
| Reviser | Cataloger |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.11.16.M.Sc.2020.Ah.E | 01010110082273000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.11.16.M.Sc.2020.Ah.E | 01020110082273000 | 22.09.2023 | CD - Rom | 82273.CD |