The possible role of using snake venom fraction(s) and gamma rays in improving snake antivenin production / (Record no. 80125)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03412cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223032712.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 210306s2020 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.08.09.Ph.D.2020.Es.P |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Esraa Mahmoud Samy Elsaied Saleh |
| 245 14 - TITLE STATEMENT | |
| Title | The possible role of using snake venom fraction(s) and gamma rays in improving snake antivenin production / |
| Statement of responsibility, etc. | Esraa Mahmoud Samy Elsaied Saleh ; Supervised Hekma A. Abdellatif , Esmat A. Shaaban , Mohammed F. Elyamany |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | الدور المحتمل لإستخدام تجزيئات سم الثعبان و آشعة جاما فى تحسين إنتاج مضاد سم الثعبان |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Esraa Mahmoud Samy Elsaied Saleh , |
| Date of publication, distribution, etc. | 2020 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 153 P. : |
| Other physical details | charts , facsimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (Ph.D.) - Cairo University - Faculty of Pharmacy - Department of Pharmacology and Toxicology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Echis pyramidum (Epy) is a venomous snake belongs to Viperidae family; it causes fetal coagulopathy systemic effects and death. Searching for more effective and safe antivenin is mandatory for viper bites treatment. Proteases are the most lethal components in viper venom inducing hemorrhage, edema and coagulation problems. Thus, the study aims to evaluate the potency of the prepared antivenin and their neutralizing activities against the biological, biochemical and histopathological activities induced by whole Epy venom individually. Echis pyramidum metalloprotease enzyme was purified using gel filtration chromatography followed by DEAE-ion exchange chromatography. The purified Epy metalloprotease enzyme (SVMP) was detoxified with 1.5 kGy gamma rays from cobalt60 gamma cell and used for immunization. 1.5 kGy irradiated Epy metalloprotease (SVMPi) showed less lethal activity (LD50) compared to the corresponding native immunogen. The prepared antivenins boosted against whole Epy venom (WV), 1.5 kGy irradiated whole Epy venom (WVi), SVMP and SVMPi were tested for neutralization of lethality and biological, biochemical and histopathological activities induced by Epy venom. The ELISA titers of the sera pool from rabbits immunized by WVi and SVMPi were higher than their corresponding native ones. The anti-SVMPi serum showed the highest neutralization of lethality (ED50) compared to the other prepared antivenins. In addition, it prolonged the clotting time from 49.0 ± 2.5 to 176.2 ± 1.4 s. Furthermore, it demonstrated a highly neutralizing activity against edema induction and hemorrhage of Epy venom by 66.8% and 94.3%, respectively compared with the other prepared antivenins. Native Epy venom was very toxic to the kidney as it produced after 4h significant increase in urea, creatinine levels, MDA, TNF-Ü, VCAM-1, IL-18, renal caspase-1, NGAL and Kim-1 contents and significant decrease in albumin level, GSH and SOD contents compared to the normal group |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Antivenin |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Chromatography |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Gamma irradiation |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Esmat A. Shaaban , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hekma A. Abdellatif , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mohammed F. Elyamany , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Shimaa |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.08.09.Ph.D.2020.Es.P | 01010110082887000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.08.09.Ph.D.2020.Es.P | 01020110082887000 | 22.09.2023 | CD - Rom | 82887.CD |