Genome-scale investigation of the regulation of azoreductase activity in representative gram-negative bacteria / (Record no. 80168)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03215cam a2200313 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 210308s2021 ua d f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.08.06.M.Sc.2021.Mo.G |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Mona Ahmed Abdelkader Mohamed Salem |
| 245 10 - TITLE STATEMENT | |
| Title | Genome-scale investigation of the regulation of azoreductase activity in representative gram-negative bacteria / |
| Statement of responsibility, etc. | Mona Ahmed Abdelkader Mohamed Salem ; Supervised Ramy K. Aziz , Abdelgawad M. Hashem |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | دراسة على نطاق الجينوم لتنظيم نشاط جينات الآزوريدكتاز فى بعض البكتريا سلبية الجرام |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Mona Ahmed Abdelkader Mohamed Salem , |
| Date of publication, distribution, etc. | 2021 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 149 P. : |
| Other physical details | charts ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Bacterial azoreductases are enzymes that decolorize azo dyes via the reductive cleavage of the azo bond. Despite the extensive studies for the identification, isolation and characterization of azoreductases from different bacteria, very minimal research was conducted on the regulation of their gene expression. Since azo dyes are man-made, the physiological roles that azoreductases might play are of particular interest.In an attempt to study the regulation of azoreductases in Escherichia coli, transcriptional data from the Gene Expression Omnibus (GEO) resource were extracted and analyzed to determine how different factors might affect the expression of azoR. Subsequently, using reverse transcription quantitative polymerase chain reaction (RT-qPCR)confirmedsome of these computational predictions. For example, increasing glucose concentration was demonstrated to decreaseazoR expression, whereas increasing culture density increased the expression. Furthermore, azoR expression was significantly higher at an incubation temperature of 30 {uF0B0}C than at 37 {uF0B0}C. Finally, azoR expression significantly decreased whenagitation speed was increased. Similarly, expression data for Pseudomonas aeruginosa were extracted from the GEO and analyzed, and the conditions affecting the expression of azoreductases and azoreductase-like genes in P. aeruginosa were predicted in silico. All genes of interest except paWrbA showed a similar predicted expression pattern at the different studied conditions: Predicted gene expression increased upon growth on minimal media, at late stationary phase with 30 {uF0B0}C incubation using relatively higher agitation speed. Co-expression analysis of azoR in different microarray studies deposited in the GEO was carried out followed by gene clustering based on their functional annotation. Nine genes namely, melA, tpx, yciK, fdnG, fpr, nfsA, nfsB, rutF, and chrR (yieF) were clustered with azoR |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Azoreductase |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Regulation |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Transposon library |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Abdelgawad M. Hashem , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ramy K. Aziz , |
| Relator term | |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Shimaa |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.08.06.M.Sc.2021.Mo.G | 01010110082912000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.08.06.M.Sc.2021.Mo.G | 01020110082912000 | 22.09.2023 | CD - Rom | 82912.CD |