Pathological, immunohistochemical and molecular studies on the curative role of synthetic cannabinoid receptors-2 agonist (AM1241) in induced hepatic and pulmonary fibrosis in male rats / (Record no. 80554)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03376cam a2200337 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223032725.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 210407s2021 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.10.05.Ph.D.2021.Al.P |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Alaa Mohamed Ali |
| 245 10 - TITLE STATEMENT | |
| Title | Pathological, immunohistochemical and molecular studies on the curative role of synthetic cannabinoid receptors-2 agonist (AM1241) in induced hepatic and pulmonary fibrosis in male rats / |
| Statement of responsibility, etc. | Alaa Mohamed Ali ; Supervised Sahar Samir Mahmoud Abdelrahman , Osama Samir Zaky Eltawil |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | فى تليف الكبد والرئة المستحدث فى ذكور الجرذان (AM1241) -2دراسات باثولوجية وامينوهيستوكيميايئية وجزيئية على الدورالعلاجى للمحفزات المصنعة لمستقبلات القنب |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Alaa Mohamed Ali , |
| Date of publication, distribution, etc. | 2021 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 137 P . : |
| Other physical details | charts , facsmilies ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Clinical Pathology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | The activation of cannabinoid receptor 2 (CB2) plays a pleiotropic role in the innate immunity and is considered a crucial mediator in liver and lung disease. This study aimed to explore the role of activating CB2 using synthetic agonist (AM1241) on suppressing the progress of liver fibrosis induced by two models; bile duct ligation (BDL) and TAA administration as well as lung fibrosis induced by bleomycin (BLM) instillation. The study also attempted to clarify the mechanisms of the antifibrotic effects of synthetic CB2 agonists; AM1241 on the ongoing both hepatic and pulmonary fibrosis. AM1241 was administrated to rats of the aforementioned three models in two doses (3 and 6 mg/kg) for 14 days (in BDL experiment) and 21days (for the other experiments). Liver function and oxidative stress markers, hepatic TNF-Ü, IL-6 and IL-10, IL-1Ý, TGF-Ý1, RT-qPCR expression of Toll like receptor 4 (TLR4), TGF-Ý1, Ü-SMA and microRNA- 155 (miR-155) genes, western blot for Vimentin and E-cadherin proteins, immunohistochemical expression of CD31, CD34, Ü-SMA and NF-mB as well as histopathology of liver tissue were all assessed. In lung experiment, miRNA-21, Myd88 and TLR4 genes expression were detected by RT-q-PCR, while collagen I, Smad2 and Myd88 protein levels were analyzed by western blot. In addition, antioxidant activity (SOD and GSH), pro-fibrotic (TGF-Ý1) and pro-inflammatory (IL- 4 and IL-13) cytokines were determined in lung tissue homogenate using ELISA kits. Histological examination and immunohistochemical (IHC) evaluation of CD4, Ü-SMA and TGF- Ý1 expression were also examined. In both liver models; AM1241 administration at both doses significantly (P<0.05) ameliorated liver function markers, pro-inflammatory cytokines (TNF-Ü, IL-6), significantly decreased i GSH content and TLR4 gene expression |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | AM1241 |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Cannabinoid receptor 2 |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | TLR4 |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Osama Samir Zaky Eltawil , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sahar Samir Mahmoud Abdelrahman , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Amira |
| Reviser | Cataloger |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.10.05.Ph.D.2021.Al.P | 01010110083145000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.10.05.Ph.D.2021.Al.P | 01020110083145000 | 22.09.2023 | CD - Rom | 83145.CD |