Preliminary investigation of Staphylococcus epidermidis exo-proteome in quest for diagnostic targets / (Record no. 80566)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03092cam a2200337 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223032725.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 210408s2021 ua dho f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.08.06.M.Sc.2021.Di.P |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Dina Mohamed Moustafa |
| 245 10 - TITLE STATEMENT | |
| Title | Preliminary investigation of Staphylococcus epidermidis exo-proteome in quest for diagnostic targets / |
| Statement of responsibility, etc. | Dina Mohamed Moustafa ; Supervised Ahmed Sherif Attia , Reham Samir |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | بحث مبدئى لدراسة البروتيوم الخارجى للمكورات العنقودية البشروية طلبًا لأهداف تشخيصية |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Dina Mohamed Moustafa , |
| Date of publication, distribution, etc. | 2021 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 112 P . : |
| Other physical details | charts , facsmilies , photographs ; |
| Dimensions | 30cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Staphylococcus epidermidis is a commensal bacterium that colonizes the skin immediately after birth protecting us from invading harmful bacteria. However, in the past decades, it was found that S. epidermidis became an opportunistic organism in patients with indwelling medical devices especially immunocompromised ones. It was noted that it is one of the most common causes of sepsis caused by biofilms in catheters. It also contributes in neonatal morbidity and mortality in the neonatal intensive care units (NICUs). Diagnosis of S. epidermidis infection takes several days by blood culturing so health-care providers use broad-spectrum antibiotics aiming to treatment but unfortunately, this might lead to the development of multiple drug resistant strains. So, we planned to find a rapid and accurate diagnostic marker for S. epidermidis. Bioinformatics analyses of the S. epidermidis proteome revealed that the N-terminal fragment of the SesK protein was found to be highly conserved and species-specific candidate. Recombinant DNA technology was used to clone, express and purify the SesK-NT polypeptide for animal immunization and hence, antibodies production. The current study utilized, for the first time, SesK-NT polypeptide to produce antibodies that could be used as a diagnostic probe for S. epidermidis. The produced antibodies could differentially distinguish between S. epidermidis and another coagulase-negative staphylococcus (S. simulans).In conclusion, this study provides preliminary evidence for the potential use of the SesK-NT protein fragment as a target for the specific detection of S. epidermidis and further optimization is required to enhance the specificity against other coagulase-negative staphylococci |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | exo-proteome in quest |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Neonatal intensive care units (NICUs) |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Staphylococcus |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ahmed Sherif Attia , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Reham Samir, |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Amira |
| Reviser | Cataloger |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.08.06.M.Sc.2021.Di.P | 01010110083252000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.08.06.M.Sc.2021.Di.P | 01020110083252000 | 22.09.2023 | CD - Rom | 83252.CD |