mazEF toxin-antitoxin system expression in methicillin resistant and methicillin sensitive staphylococcus aureus and its relation to biofilm-formation / (Record no. 80684)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03380cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223032729.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 210417s2021 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.11.19.Ph.D.2021.Ay.M |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Aya Bakr Elesawi |
| 245 10 - TITLE STATEMENT | |
| Title | mazEF toxin-antitoxin system expression in methicillin resistant and methicillin sensitive staphylococcus aureus and its relation to biofilm-formation / |
| Statement of responsibility, etc. | Aya Bakr Elesawi ; Supervised Heba Hamed Arnaout , Yasmine Samy Elkholy , Rania Yahia Shash |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | السم ومضاد السم بين الكريات العنقودية الذهبية المقاومة و الحساسة للميثيسيلين وعلاقته بتكوين غشاء حيوى mazEF مقارنة نواتج النظام الجينى |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Aya Bakr Elesawi , |
| Date of publication, distribution, etc. | 2021 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 105 P. : |
| Other physical details | charts , facsimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Microbiology and Immunology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Background: Staphylococcus aureus is a common cause of many healthcare-associated and community-acquired infections. Methicillin resistance in S. aureus has become prevalent worldwide. Moreover biofilm-formation makes it more difficult to eradicate bacteria by antibiotics. mazEF toxin antitoxin system consists of a stable toxin called mazF which acts as an endoribonuclease and a labile antitoxin called mazE which opposes the toxin action. mazEF genes are located on S. aureus chromosome and can be used as an alternative target in treatment of infections. Objectives: The aim of the present work was to detect mazEF expression in MRSA isolates compared to MSSA isolates as well as determining mazEF system expression in relation to biofilm-formation. Methods: One hundred non-duplicate S. aureus isolates were collected from patients admitted to Kasr Al-Aini University Hospitals and categorized into 50 MRSA and 50 MSSA. All isolates were tested for antimicrobial susceptibility using disk diffusion method and biofilm-formation using tissue culture plate method. Finally, mazEF expression was detected by real time PCR and correlated to both methicillin susceptibility and biofilm-formation. Results: MRSA isolates were shown to be more resistant to antimicrobial agents tested than MSSA isolates. Linezolid and gentamycin showed the best sensitivity results with both MRSA and MSSA. 88% of MRSA and 96% of MSSA isolates were sensitive to linezolid while 76% of MRSA and 84% of MSSA isolates were sensitive to gentamycin. Biofilm-formation was statistically significantly higher in MRSA isolates than MSSA isolates (p value = 0.037). Expression of mazEF was statistically significantly higher in MRSA isolates compared to MSSA isolates (p value < 0.001), but no statistically significant correlation was detected between mazEF expression and biofilm-formation |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | MRSA |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | MSSA |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Toxin-antitoxin systems |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Heba Hamed Arnaout , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Rania Yahia Shash , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Yasmine Samy Elkholy , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Shimaa |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.11.19.Ph.D.2021.Ay.M | 01010110083280000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.11.19.Ph.D.2021.Ay.M | 01020110083280000 | 22.09.2023 | CD - Rom | 83280.CD |