Synthetic lethality of triple negative breast cancer through trgeted silencing by small interfering RNA / (Record no. 80960)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03194cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223032738.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 210520s2021 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.08.01.Ph.D.2021.Ma.S |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Mahmoud Taha Abdelaziz Aboelfadl |
| 245 10 - TITLE STATEMENT | |
| Title | Synthetic lethality of triple negative breast cancer through trgeted silencing by small interfering RNA / |
| Statement of responsibility, etc. | Mahmoud Taha Abdelaziz Aboelfadl ; Supervised Manal Fouad Ismail , Nancy Nabil Shahin , Amira M. Gamal Aldeen |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | الموت الاصطناعى لسرطان الثدى ثلاتى السلبية من خلال الاسكات الموجه باستخدام الحمض النووى الريبوزى المتداخل الصغير |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Mahmoud Taha Abdelaziz Aboelfadl , |
| Date of publication, distribution, etc. | 2021 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 262 P . : |
| Other physical details | charts facsmilies ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (Ph.D.) - Cairo University - Faculty of Pharmacy - Department of Biochemistry |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Background: TNFRSF13B, TACI, is a member of the TNF receptor superfamily; it plays a key role in cancer cell proliferation and progression. Method: Influence of silencing of human cytokine receptors on cell viability was screened by Luminescent Cell Viability Assay, after transfection with the siRNA library to find the maximum cell death superhits in both triple- negative MDAMB- 231 and triple-positive MCF-7 breast cells. The mode of cell death was investigated by dual DNA fluorescence staining. The expression of mRNAs of TACI, BAFF, BAFF-R, and APRIL was explored by qPCR. Immunocytofluorescence analysis was used to evaluate changes in TACI, Bcl-2, TNFR2, cyclin-D2, and PCNA. NF-kB p65, cell cycle, and necrosis/apoptosis (late and early) were analyzed by flowcytometry. Results: TACI was the most potent cytotoxic superhit resulting from highthroughput screening of the siRNA library, in both types of cells. Our findings indicated that silencing TACI receptor in both types of breast cancer cells led to significant cell death, after different intervals from siRNA transfection. Cell death mediators (TNF-R2, Bcl-2, and NF-mB p65) were significantly decreased after TACI silencing. The key factors for cell division (cyclin-D2 and PCNA) were significantly increased in silenced cells of both types but the cell cycle was arrested before the completion of mitosis. Expression of BAFF, BAFF-R and APRIL mRNA in TACI-silenced cells showed significant upregulation in MDAMB- 231 cells, while only BAFF-R and APRIL showed significant downregulation in MCF-7 cellsConclusion: TACI silencing can be a new and promising therapeutic strategy for mesenchymal-stem like triple- negative breast cancer subtype |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | RNA |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Synthetic lethality |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Triple negative breast cancer |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Amira M. Gamal Aldeen, |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Manal Fouad Ismail , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Nancy Nabil Shahin , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Amira |
| Reviser | Cataloger |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.08.01.Ph.D.2021.Ma.S | 01010110083466000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.08.01.Ph.D.2021.Ma.S | 01020110083466000 | 22.09.2023 | CD - Rom | 83466.CD |