Assessment of anti-cancerous effect of green, roasted and decaffeinated coffee on oral squamous cell carcinoma cell line : (Record no. 81074)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03114cam a2200337 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223032742.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 210529s2020 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.09.14.Ph.D.2020.As.A |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Asmaa Emad Eldin Mohammed Rashad |
| 245 10 - TITLE STATEMENT | |
| Title | Assessment of anti-cancerous effect of green, roasted and decaffeinated coffee on oral squamous cell carcinoma cell line : |
| Remainder of title | In vitro study / |
| Statement of responsibility, etc. | Asmaa Emad Eldin Mohammed Rashad ; Supervised Mohsen Kazem Abdellatif , Manar Abdulwaniss Mohammed |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | تقييم التأثير المضاد للسرطان للبن الأخضر والمحمص والمنزوع منه الكافيين على خط خلية سرطان الخلايا الحرشفية : |
| Remainder of title | دراسة مختبرية غير عشوائية |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Asmaa Emad Eldin Mohammed Rashad , |
| Date of publication, distribution, etc. | 2020 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 120 P. : |
| Other physical details | charts , facsimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (Ph.D.) - Cairo University - Faculty of Oral and Dental Medicine - Department of Oral and Maxillofacial Pathology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Aim: The aim of our study to investigate and compare the effect of green, medium roasted and decaffeinated coffee on the viability, apoptosis, cell cycle progression, proliferation, invasion and migration of OSCC cell lines. Methodology: Coffee extracts were prepared and oral squamous cell carcinoma cultures were exposed to various concentrations of coffee extracts for 72 hr.Cell viability was assessed and IC50 values for each extract were quantified using MTT assay. Using flowcytometry, apoptotic and necrotic cell population were calculated followed by the assessment of caspase-3 and caspase-9 activity. Cell cycle analysis for each coffee extracts was conducted. Next, EGFRTK assay was done to obtain the inhibition % of tyrosine kinase activity in the studied groups. We assessed the antimigration and anti-invasion effect of coffee extracts on oral squamous cell carcinoma cells using Transwell invasion and migration assay. Finally, HPLC analysis was performed to measure the amount of total phenolic, chlorogenic acid and caffeine in coffee extracts. Results: The results showed a presence of cytotoxic effect of coffee extracts and the IC 50 was 17.1±0.69, 57.95±2.28, and 103.32 ±4.52 in green coffee, roasted coffee and decaffeinated coffee respectively. The total apoptotic and necrotic cell populations was increased in green coffee followed by roasted then decaffeinated compared to control. Capsase-3 and caspase-9 activity was up-regulated in all coffee extract groups with the same order. All coffee extracts arrested cell cycle at G2-M phase |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Anti-cancerous |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Carcinoma cell line |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Decaffeinated coffee |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Manar Abdulwaniss Mohammed , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mohsen Kazem Abdellatif , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Shimaa |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.09.14.Ph.D.2020.As.A | 01010110083534000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.09.14.Ph.D.2020.As.A | 01020110083534000 | 22.09.2023 | CD - Rom | 83534.CD |