Studies on the conversion of Tetanus toxin to its corresponding toxoid and conditions affecting its reversion / (Record no. 81138)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03507cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223032744.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 210601s2021 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.08.06.M.Sc.2021.Ma.S |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Marwa Mohammed Reda Abdelazim |
| 245 10 - TITLE STATEMENT | |
| Title | Studies on the conversion of Tetanus toxin to its corresponding toxoid and conditions affecting its reversion / |
| Statement of responsibility, etc. | Marwa Mohammed Reda Abdelazim ; Supervised Mohammed Abdelhaleem Ramadan , Aymen Samir Mohammed Yassin , Mohammed Elsayed Ali Rashed |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | دراسات عن تحويل سم التيتانوس إلى مقابله الغيرسام و الظروف المؤثرة على الارتداد للسُمية |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Marwa Mohammed Reda Abdelazim , |
| Date of publication, distribution, etc. | 2021 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 105 P . : |
| Other physical details | charts , facsmilies ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Tetanus is one of the major causes of extensive morbidity and mortality in rural areas of developing countries affecting both animals and humans. The commercially available tetanus vaccine is routinely produced through detoxification of the tetanus toxin using formaldehyde followed by purification process, which might show residual formaldehyde presence and some reversion to toxic form upon exposure to variable conditions. In the current study, in an approach to optimize the production conditions and to minimize the risk of crosslinking of unwanted foreign proteins during the detoxification processes; switching the order of the purification and the detoxification processes was tested. The bacterial crude toxin was purified at optimized conditions utilizing 40% ammonium sulfate and incubated for seven days followed by successive dialysis steps. SDS PAGE using both Coomassie brilliant blue and silver staining, as well as HPLC techniques were used to confirm the integrity and the purity of the produced toxins. Detoxification of the purified tetanus toxin was tested by using gradient decreasing concentrations of formaldehyde; where complete detoxification was achieved utilizing 0.08% with five weeks dark incubation at 37{u00B0}C. Another detoxification technique was demonstrated using iodoacetamide buffer as a reducing agent instead of formaldehyde, where complete detoxification was achieved within five hours using dark incubation at 37{u00B0}C, followed by dialysis to remove excessive salts. Comparative analysis was carried out on the newly prepared toxoid versus the traditionally produced one to evaluate the efficacy and safety as well as to monitor the rate and extent of reversion to toxicity. The obtained results proved that iodoacetamide detoxification was faster than the commonly used formaldehyde method, and it produced similar survival rates in animals{u2019} models, also the new preparation was more potent at dilutions (1/5 &1/10) and induced higher protective antibody levels in mice sera |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Purification |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Tetanus toxin |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Toxoids |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Aymen Samir Mohammed Yassin , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mohammed Abdelhaleem Ramadan , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mohammed Elsayed Ali Rashed , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Amira |
| Reviser | Cataloger |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.08.06.M.Sc.2021.Ma.S | 01010110083562000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.08.06.M.Sc.2021.Ma.S | 01020110083562000 | 22.09.2023 | CD - Rom | 83562.CD |